文献类型：期刊文献

英文题名：Phenotyping of Methicillin-Resistant Staphylococcus aureus Using a Ratiometric Sensor Array

作者：Hu, Xi-Le[1,2];Gan, Hui-Qi[1,2];Qin, Zhao-Yang[1,2];Liu, Qian[3];Li, Min[3];Chen, Daijie[4];Sessler, Jonathan L.[5];Tian, He[1,2];He, Xiao-Peng[1,2]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Joint Int Res Lab Precis Chem, Shanghai 200237, Peoples R China;[3]Ren Ji Hosp Eastern, Dept Lab Med, Shanghai 200127, Peoples R China;[4]Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China;[5]Univ Texas Austin, Dept Chem, Austin, TX 78712 USA

年份：2023

卷号：145

期号：16

起止页码：8917

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20231613900071);WOS:【SCI-EXPANDED(收录号:WOS:000972008700001)】；

基金：The authors thank the Natural National Science Foundation of China (NSFC) (No. 92253306 to X.-P.H., 21907030 to X.- L.H., 81872775 to D.C., and 82072235 to M.L.) , the Shanghai Municipal Science and Technology Major Project (No. 2018SHZDZX03 to X.-P.H. and H.T.) , the Fundamental Research Funds for the Central Universities (222201717003 to X.-P.H. and H.T.) , and the Program of Introducing Talents of Discipline to Universities (B16017 to H.T.) for financial support and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering. The work in Austin was supported by the Robert A. Welch Foundation (F-0018 to J.L.S.) . The Research Center of Analysis and Test of East China University of Science and Technology was gratefully acknowledged for assistance in analytical experiments.

语种：英文

外文关键词：Bacteria - Diagnosis - Fluorescence - Polymerase chain reaction - Principal component analysis - Probes - Salts

摘要：Chemical tools capable of classifying multidrugresistant bacteria (superbugs) can facilitate early-stage disease diagnosis and help guide precision therapy. Here, we report a sensor array that permits the facile phenotyping of methicillinresistant Staphylococcus aureus (MRSA), a clinically common superbug. The array consists of a panel of eight separate ratiometric fluorescent probes that provide characteristic vibration-induced emission (VIE) profiles. These probes bear a pair of quaternary ammonium salts in different substitution positions around a known VIEgen core. The differences in the substituents result in varying interactions with the negatively charged cell walls of bacteria. This, in turn, dictates the molecular conformation of the probes and affects their blue-to-red fluorescence intensity ratios (ratiometric changes). Within the sensor array, the differences in the ratiometric changes for the probes result in "fingerprints" for MRSA of different genotypes. This allows them to be identified using principal component analysis (PCA) without the need for cell lysis and nucleic acid isolation. The results obtained with the present sensor array agree well with those obtained using polymerase chain reaction (PCR) analysis.