文献类型：期刊文献

英文题名：Dynamic Metabolism Mechanism of Halomonas Elongata Dsm 2581t Response to Nacl Shock Stress: An Effective Technology for Enhancing Ectoine Biosynthesis

作者：Yu, Junxiong[1]; Zhang, Yue[1]; Wang, Jing[2]; Liu, Yuxuan[2]; Mohisn, Ali[1]; Liu, Hao[1]; Zheng, Yanning[3]; Xing, Jianmin[4]; Zhuang, Yingping[1]; Guo, Meijin[1]; Wang, Zejian[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai, 200237, China; [2] Department of Chemical Engineering for Energy Resources, East China University of Science and Technology, 130 Meilong Rd, Shanghai, 200237, China; [3] State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing, 100101, China; [4] College of Chemical Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230261167)

语种：英文

外文关键词：Biochemistry - Biosynthesis - Industrial research - Industrial water treatment - Metabolism - Metabolites - Wastewater treatment

摘要：Halophiles are key microorganisms used in industrial biotechnology, with high value and intense research focus. This study investigated the stress response of Halomonas elongata DSM 2581T to NaCl shock. Results showed that tolerable NaCl shock treatments greatly stimulated ectoine accumulation, reaching maximum productivity (1450 ± 99 mg/L/h) and specific production rate (66 mg ectoine/g DCW/h) at 8% NaCl shock. However, NaCl shock significantly inhibited cell respiration, aggravating with the increased NaCl concentration. Furthermore, metabolite analysis revealed that intracellular amino acids and stored sugar contents exhibited a faster response compared to ectoine within 30 mins after shock. Transcriptome profiling showed upregulation of sulfur metabolism and flagellar assembly pathways, but no enhancement in ectoine biosynthesis within 10 minutes after shock. Moreover, antioxidants and osmoprotectants effectively protected cells from shock treatments. This study enhances the understanding of stress response of H. elongata to NaCl shock and provides possible utilization for treating hypersaline wastewater. ? 2023, The Authors. All rights reserved.