文献类型：期刊文献

中文题名：广藿香精油抗炎祛痘功效研究

英文题名：Anti-acne efficacy of essential oil from Pogostemon cablin(Blanco) Benth.

作者：王玥[1,2,3];郭苗苗[2];施雁勤[1];潘仙华[1];卢艳花[2];曹平[3]

机构：[1]上海应用技术大学,上海201418;[2]华东理工大学生物反应器工程国家重点实验室,上海200237;[3]上海家化(集团)有限公司,上海200082

年份：2017

卷号：47

期号：5

起止页码：272

中文期刊名：日用化学工业

外文期刊名：China Surfactant Detergent & Cosmetics

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2017_2018】；

基金：教育和科研协同创新计划--上海市香料香精及化妆品协同创新中心资助项目(1021ZK162001)

语种：中文

中文关键词：化妆品添加剂;广藿香精油;抗炎祛痘;痤疮丙酸杆菌

外文关键词：cosmetic additive ; essential oil from Pogostemon cablin ; anti -acne ; Propionibacterium aches

摘要：采用气相色谱-质谱联用技术对广藿香精油成分进行分析。通过细胞毒性实验确定广藿香精油对人急性单核细胞(THP-1)的安全质量浓度。利用痤疮丙酸杆菌(P.acnes)刺激THP-1建立的特异性痤疮炎症细胞模型考察广藿香精油对炎症因子IL-1β的影响。通过荧光实时定量逆转录聚合酶链反应(qRT-PCR)初步探讨广藿香精油抗炎祛痘的作用机制。研究结果表明:广藿香精油在最大安全质量浓度12.5 mg/L时,对炎症细胞模型中P.acnes活性影响较小,对IL-1β的分泌具有明显的抑制作用(抑制率66.54%),可显著降低细胞内Toll样受体2(TLR2)mRNA的转录(抑制率73.73%),从而起到抑制炎症信号转导和继发性炎症介质生成的作用。
Chemical composition of the essential oil from Pogostemon cablin （Blanco） Benth. （EOP） was quantitatively analyzed by gas chromatography - mass spectrometry （ GC - MS）. The safe mass concentration of the EOP to Human Acute Mononuclear Cells （ THP - 1 ） was determined via cytotoxicity screening test. An inflammatory ache model was established by stimulating THP - 1 with Propionibacterium aches （P. aches ）. Subsequently, the effect of the EOP on the expression of proinflammatory cytokine IL - 1 β was assessed. The anti -acne mechanism of the EOP was examined by qRT - PCR. Results show that at maximum safe mass concentration of 12. 5 mg/L of the EOP, the influence on the activity of P. aches is rather small and the secretion of IL - 1 β is significantly inhibited by a rate of 66.54%. The EOP also significantly reduces the mRNA level of Toll- like receptor 2 （TLR2） by 73.73%. Thereby,the EOP inhibits proinflammatory signal transduction and production of secondary inflammatory mediators.