文献类型：期刊文献

英文题名：Analysis of metabolic fluxes for better understanding of mechanisms related to lipid accumulation in oleaginous yeast Trichosporon cutaneum

作者：Liu, Zhijie[1]; Gao, Yang[1]; Chen, Jun[1]; Imanaka, Tadayuki[1,2]; Bao, Jie[1]; Hua, Qiang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [2] Department of Biotechnology, College of Life Sciences, Ritsumeikan University, Kusatsu 525-8577, Japan

年份：2013

卷号：130

起止页码：144

外文期刊名：Bioresource Technology

收录：EI(收录号：20130315911015)

语种：英文

外文关键词：Yeast - Biodiesel - Metabolism - Sulfur compounds - Nitrogen compounds - Enzymes

摘要：Microbial fermentation for producing biodiesel from lignocellulosic hydrolysates is receiving increasing attention and attempts have been made to screen an oleaginous Trichosporon sp. with high lipid content and a strong tolerance to lignocellulose hydrolysates. In order to better understand mechanisms related to its lipid accumulation, metabolic flux analysis was performed under 5gL-1 ammonium sulfate (high nitrogen) and/or 0.4gL-1 ammonium sulfate (low nitrogen) conditions. Cell growth phase and lipid accumulation phase were shown for cells grown under low nitrogen condition. Results of flux distribution demonstrated that NADPH provided by cytosolic malic enzyme and the acetyl-CoA from cytoplasmic citrate by the ATP: citrate lyase were the two primary sources for excess lipid accumulation. Flux data also supported the fact that the citrate pyruvate cycle plays an essential role in the lipid accumulation. The flux information obtained could also motivate new design strategies for oleaginous yeasts for enhanced biodiesel production. ? 2012 Elsevier Ltd.