文献类型：期刊文献

英文题名：Process development of succinic acid production by Escherichia coli NZN111 using acetate as an aerobic carbon source

作者：Liu, Yuan[1]; Wu, Hui[1]; Li, Qing[1]; Tang, Xuwei[1]; Li, Zhimin[1]; Ye, Qin[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China

年份：2011

卷号：49

期号：5

起止页码：459

外文期刊名：Enzyme and Microbial Technology

收录：EI(收录号：20114214446116)

语种：英文

外文关键词：Organic acids - Productivity - Carbon - Escherichia coli - Batch data processing

摘要：Escherichia coli strain NZN111 could convert glucose to succinic acid efficiently in anaerobic conditions after the induction of gluconeogenic carbon sources in aerobic conditions. Acetate shows a strong effect on both yield and productivity of succinic acid. In this study, the fed-batch process of succinic acid production by NZN111 using acetate in a chemically defined medium in the aerobic stage was investigated and developed. Increasing cell density could increase succinic acid with a productivity of 3.97. g/(L. h) in the first 8. h of the anaerobic phase with an overall yield of 1.42. mol/mol glucose in a 5. L fermentor. However, there was strong repression from succinic acid in the later anaerobic stage. When succinic acid exceeded 30. g/L, the glucose consumption rate began to drop sharply along with the succinic acid production rate. Supplementation with glucose from 30 to 70. g/L in the anaerobic stage showed little effect on succinic acid production. Acetic acid and pyruvic acid accumulated had no effect on succinic acid formation because of their low concentration. With acetate as the sole carbon source for aerobic cultivation in the following scale-up, 60.09. g/L of succinic acid was produced with a yield of 1.37. mol/mol in a 50. L bioreactor. ? 2011 Elsevier Inc.