文献类型：期刊文献

中文题名：大孔弱酸树脂对卡那霉素吸附行为的研究

英文题名：STUDY ON THE EXTRACTION CHARACTERISTICS OF KANAMYCIN ON THE MACRORETICULAR WEAK ACID ION EXCHANGE RESIN

作者：严希康[1];赵丽[1];仇月霜[1];杨雅琴[1];沈林南[1]

机构：[1]华东化工学院生化工程研究所,上海200237

年份：1993

卷号：18

期号：4

起止页码：278

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心1992】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：弱酸树脂;卡那霉素;离子交换法

外文关键词：Macroreticular ion exchange resin; Kanamycin; Sorption capacity; Eluate

摘要：本文比较了大孔弱酸树脂和强酸732树脂对卡那霉素的吸附行为,包括交换容量和洗脱性能。用正交设计试验法和电子计算机理论演算.确定了最适工艺条件,最后还用发酵液进行了动态试验。实验表明华化大孔弱酸-Ⅲ号树脂对卡那霉素的交换容量,不仅高于732树脂22％,同样比Amberlite IRC-50树脂也高,在滤液呈中性条件下,浓度为8000u/ml左右时吸附,用2.5％氨水解吸,可获得卡那霉素离子交换过程最佳状态,回收率增加、浓缩倍数提高、能耗降低。
The extraction characteristics such as sorption capacity and elution property etc. of Kanamycin on the macroreticular weak acid ion exchange reisn and the conventional gel-type strong acid ion exchange resin 732 were compared. The optimum process of the extraction by macroreticular ion exchanger was studied by means of orthogonal analytical method and theoretical calculation with the aid of computer. Based on the following conditions:concentration of Kanamycin filtrate is 8,000u/ml, neutral pH, and using 2.5% of ammonia as the eluent, the column performances with fermentation broth filtrate were carried out. Results showed that the sorption capacity of the Hua Hua Ⅲ macroreticular weak acid ion exchange resin possesses a higher sorption capacity than the conventional gel-type strong acid ion exchange resin 732 and the Amberlite 150. The overall yield and the concentration of the eluate of Kanamycin are increased and the purpose of reducing energy consumption is also attained.