文献类型：期刊文献

英文题名：Enhancing gluconic acid production by controlling the morphology of Aspergillus niger in submerged fermentation

作者：Lu, Fei[1];Ping, Kangkang[1];Wen, Ling[1];Zhao, Wei[2];Wang, Zejian[1];Chu, Ju[1];Zhuang, Yingping[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shan Dong Fuyang Biol Technol Co Ltd, Dezhou, Shandong, Peoples R China

年份：2015

卷号：50

期号：9

起止页码：1342

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20152300920790);WOS:【SCI-EXPANDED(收录号:WOS:000359166400002)】；

基金：This work was financially supported by grants from Major State Basic Research Development Program of China (973 Program) (No. 2013CB733600 and 2012CB72100x), National High Technology Research and Development Program (2015AA021005) and the National Natural Science Foundation of China (Grant No. 31200024). We also thank Shan Dong Fuyang Biological Technology Co., Ltd (DeZhou, China) for donating the industrial strain of A. niger.

语种：英文

外文关键词：Gluconic acid; Aspergillus niger; Morphology; Oxygen transfer rate; Diffusion limitation

摘要：Effects of seed morphology of Aspergillus niger (A. niger) AN151 on gluconic acid (GA) biosynthesis were investigated in 50-L fermenters. Online physiological parameters showed that the optimum seed morphology for GA biosynthesis was dispersed pattern rather than pellets which were widely used in industrial production. The highest overall yield of 1.051 +/- 0.012 (gg(-1)) was achieved using dispersed seed in shorter fermentation time (14.5h) with an initial glucose solution of 330 g L-1, and was 3.1% higher than the best reported data. And the average GA production rate under dispersed fermentation (21.0 +/- 0.9 gL(-1) h(-1)) was 73.6% and 39.1% higher than those with large and small pellets, respectively. The fermentation results showed that conversion of mycelial morphology from pellets to dispersed state had negligible impact on viscosity of fermentation broth at low biomass concentration (1.7 g L-1), while coefficient of volumetric oxygen transfer (k(L)a) improved more than 13%, suggesting that the promotion of oxygen transfer rate (OTR) between liquid medium and mycelia greatly increased GA production. Therefore, controlling the morphology of A. niger is an effective method for improving industrial GA production. (C) 2015 Elsevier Ltd. All rights reserved.