文献类型：期刊文献

英文题名：Advancing recombinant protein expression in Komagataella phaffii: opportunities and challenges

作者：Lv, Wen[1];Cai, Menghao[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2025

卷号：25

外文期刊名：FEMS YEAST RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001459431900001)】；

基金：This work was financially supported by the National Key Research and Development Program of China (2022YFC2805102) and Shanghai Explorer Research Plan (24TS1411600).

语种：英文

外文关键词：Komagataella phaffii; non-conventional yeast; protein expression regulation; advanced methods; biotechnology applications

摘要：Komagataella phaffii has gained recognition as a versatile platform for recombinant protein production, with applications covering biopharmaceuticals, industrial enzymes, food additives, etc. Its advantages include high-level protein expression, moderate post-translational modifications, high-density cultivation, and cost-effective methanol utilization. Nevertheless, it still faces challenges for the improvement of production efficiency and extension of applicability. This review highlights the key strategies used to facilitate productivity in K. phaffii, including systematic advances in genetic manipulation tools, transcriptional and translational regulation, protein folding and secretion optimization. Glycosylation engineering is also concerned as it enables humanized glycosylation profiles for the use in therapeutic proteins and functional food additivities. Omics technologies and genome-scale metabolic models provide new insights into cellular metabolism, enhancing recombinant protein expression. High-throughput screening technologies are also emphasized as crucial for constructing high-expression strains and accelerating strain optimization. With advancements in gene-editing, synthetic and systems biology tools, the K. phaffii expression platform has been significantly improved for fundamental research and industrial use. Future innovations aim to fully harness K. phaffii as a next-generation cell factory, providing efficient, scalable, and cost-effective solutions for diverse applications. It continues to hold promise as a key driver in the field of biotechnology. This minireview highlights advancements in Komagataella phaffii expression systems, focusing on genetic engineering, transcriptional and translational regulation, protein folding and secretion optimization, omics technologies, and high-throughput screening, establishing its potential as a next-generation biotechnological platform.