文献类型：期刊文献

英文题名：The effects of PTBP3 silencing on the proliferation and differentiation of MKN45 human gastric cancer cells

作者：Chen, Bin[1];Zhao, Ai-Guang[1];Shao, Jin[2,3,4];Mu, Xiao-Yan[1];Jiang, Lin[2,3,4];Liu, Jian-Wen[2,3,4]

机构：[1]Shanghai Univ Tradit Chinese Med, Longhua Hosp, Dept Oncol, Shanghai 200032, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[4]E China Univ Sci & Technol, Dept Mol & Cellular Pharmacol, Shanghai 200237, Peoples R China

年份：2014

卷号：114

期号：1

起止页码：29

外文期刊名：LIFE SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000342273600005)】；

基金：This study was supported through grants from the National Natural Science Foundation of China (no. 81373861), the Shanghai Talents Training Program of Health System (no. XBR2013103), Program of the "Dragon Medical Team and Dragon Medical Clinician" in the National Clinical Research Base of Traditional Chinese Medicine (no. LYTD-05).

语种：英文

外文关键词：PTBP3; Gastric cancer differentiation; Proliferation

摘要：Aims: PTBP3 overexpression inhibits the differentiation of leukemia cells; however, its effects on the differentiation and proliferation of solid cancer cells remain unclear. Thus, the impact of PTBP3 on the differentiation and proliferation of gastric cancer cells was investigated. Main methods: PTBP3 expression was analyzed in normal and tumor tissues using immunohistochemistry. A xenograft model was established in nude mice by subcutaneous injection of untransfected human gastric cancer MKN45 cells or those expressing a control vector or PTBP3 siRNA. We analyzed the tumor inhibition rate, the expression of PTBP3, the PCNA-positive rate and the serum levels of CEA, CA199, CA125, LDH, ALP and gamma-GT in different groups. Key findings: The tumor weights in the PTBP3 siRNA group were significantly lower than that of the MKN45 cell control group (P < 0.001). Immunohistochemistry analysis of PCNA expression revealed that it was markedly reduced after PTBP3 silencing. ELISAs showed that the serum levels of CEA and CA199 tumor markers as well as LDH and ALP were reduced after PTBP3 silencing. Transmission electron microscopy revealed that MKN45 cells expressing PTBP3 siRNA had reduced nuclear-to-cytoplasmic ratio and regular nuclei, suggesting differentiation. Significance: PTBP3 may promote proliferation and inhibit the differentiation of human gastric cancer MKN45 cells. (C) 2014 Elsevier Inc. All rights reserved.