文献类型：期刊文献

英文题名：Polyvinyl alcohol, N-acetylcysteine, and methyl-β-cyclodextrin exhibit albumin functions in natural killer cell culture

作者：Xu, Ruisheng[1]; Zhang, Guofeng[1]; Huang, Huimin[1]; Zhao, Yuanyuan[1]; Tan, Wen-Song[1]; Cai, Haibo[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

年份：2025

卷号：139

期号：6

起止页码：436

外文期刊名：Journal of Bioscience and Bioengineering

收录：EI(收录号：20251318120901)

语种：英文

外文关键词：Animal cell culture - Body fluids - Fatty acid methyl ester - Mitochondria - Plant cell culture - Reactive oxygen species

摘要：Albumin is a crucial component of serum-free media, playing a significant role in ex vivo cell culture as a lipid carrier and antioxidant. However, purified albumin contains undefined substances, making it challenging to achieve clinical application standards for effector cell culture. This study used natural killer (NK)-92 cells as a model to investigate the effects of the albumin substitute replacing bovine serum albumin (BSA) on cell expansion and metabolism in an in-house-designed, chemically defined, serum-free medium. We selected polyvinyl alcohol (PVA), N-acetylcysteine (NAC), and methyl-β-cyclodextrin (M-β-CD) as an albumin substitute combination and optimized their concentrations by using response surface methodology. The optimized albumin substitute was named PVA-NAC-M-β-CD (PNM). After 8 days of culture, NK-92 cells cultured with the PNM exhibited phenotype and cytotoxic function comparable to cells cultured with different concentrations of BSA. The expansion fold was 89.22 ± 3.55, significantly higher than the 51.23 ± 6.57 observed in the 0.75 g/L BSA group (p ? 2025 The Society for Biotechnology, Japan