文献类型：期刊文献

英文题名：scCO 2 Foamed Composite Scaffolds Incorporating Bioactive Lipids Promote Vascularized Bone Regeneration Via Hif-1α Upregulation and Enhanced Type H Vessel Formation

作者：Li, Shuang[1]; Song, Chaobo[2]; Yang, Shengbing[4]; Yu, Weijun[3]; Zhang, Weiqi[1]; Zhang, Guohua[1]; Xi, Zhenhao[2]; Lu, Eryi[1]

机构：[1] Department of Stomatology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 160 Pujian Road, Shanghai, China; [2] Shanghai Key Laboratory of Multiphase Materials Chemical Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, China; [3] College of Stomatology, School of Medicine, Shanghai Jiao Tong University, 390 Yanqiao Road, Shanghai, China; [4] Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, 639 Zhizaoju Road, Shanghai, China

年份：2019

外文期刊名：SSRN

收录：EI(收录号：20210257787)

语种：英文

外文关键词：Bioactive glass - Bone - Carbon dioxide - Defects - Lipids - Scaffolds (biology) - Supercritical fluid extraction

摘要：Bone tissue engineering has substantial potential for the treatment of massive bone defects; however, efficient vascularization coupled with bone regeneration still remains a challenge in this field. In the current study,supercritical carbon dioxide (scCO2) foaming technique was adopted to fabricate mesoporous bioactive glasses (MBGs) particle-poly (lactic-co-glycolic acid) (PLGA) composite scaffolds with appropriate mechanical and degradation properties as well as in vitro bioactivity. The MBG-PLGA scaffolds incorporating the bioactive lipid FTY720 (designated as FTY/MBG-PLGA) exhibited simultaneously sustained release of thebioactive lipid and ions. In addition to providing a favorable microenvironment for cellular adhesion and proliferation, FTY/MBG-PLGA scaffolds significantly facilitated the in vitro osteogenic differentiation of rBMSCs and also markedly stimulated the up regulation of Hif-1α expression via the activation of the Erk1/2 pathway, which mediated the osteogenic and pro-angiogenic effects on rBMSCs. Furthermore, FTY/MBG-PLGA extracts induced superior in vitro angiogenic performance of HUVECs. In vivo evaluation of critical-sized rat calvarial bone defects indicated that FTY/MBG-PLGA scaffolds potently promoted vascularized bone regeneration. Notably, the significantly enhanced formation of type Hvessels (CD31hiEmcnhineo-vessels) was observed in newly formed bone tissue in FTY/MBG-PLGA group, strongly suggesting that FTY720 and therapeutic ions released from the scaffolds synergistically induced moretype H vessel formation, which indicated the coupling of angiogenesis and osteogenesis to achieve efficiently vascularized bone regeneration. Overall,the results indicated that the foamed porous MBG-PLGA scaffolds incorporating bioactive lipids achieved desirable vascularization-coupled bone formation and could be a promising strategy for bone regenerative medicine. ? 2019, The Authors. All rights reserved.