文献类型：期刊文献

英文题名：A short-chain dehydrogenase plays a key role in cellulosic D-lactic acid fermentability of Pediococcus acidilactici

作者：Qiu, Zhongyang[1,2];Fang, Chun[1];Gao, Qiuqiang[1];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Huaiyin Normal Univ, Jiangsu Key Lab Biomass Based Energy & Enzyme Tec, 111 West Changjiang Rd, Huaian 223300, Jiangsu, Peoples R China

年份：2020

卷号：297

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20195107874929);WOS:【SCI-EXPANDED(收录号:WOS:000504829800016)】；

基金：This research was supported by the National Natural Science Foundation of China (31961133006, 21978083) and the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (19KJB180012).

语种：英文

外文关键词：Lignin derived inhibitors; Biodetoxification; Pediococcus acidilactici; D-lactic acid; Lignocellulose

摘要：Phenolic aldehydes from lignocellulose pretreatment are strong inhibitors of cell growth and metabolism of cellulosic lactic acid bacteria. Their low solubility and recalcitrance highly reduce the removal efficiency of various detoxification methods. This study shows a simultaneous conversion of phenolic aldehydes and fermentation of D-lactic acid by Pediococcus acidilactici using corn stover feedstock. Vanillin was found to be the strongest phenolic aldehyde inhibitor to P. acidilactici. The overexpression of a short-chain dehydrogenase encoded by the gene CGS9114_RS09725 from Corynebacterium glutamicum was identified to play a key role in D-lactic acid fermentability of P. acidilactici. The engineered P. acidilactici with the genome integration of CGS9114_RS09725 showed the accelerated vanillin reduction and improved cellulosic D-lactic acid production. This study reveals that vanillin conversion is crucial for D-lactic acid fermentation, and the direct expression of a specific vanillin reduction gene in lactic acid bacterium efficiently improves cellulosic D-lactic acid production.