文献类型：期刊文献

中文题名：灵芝菌丝体中一个三萜类化合物的核磁归属及活性初探

英文题名：NMR attribution and bioactivity evaluation of a triterpene in mycelia of Ganoderma lingzhi

作者：朱晓璐[1];岳亚文[1];张劲松[2];冯杰[2];唐庆九[2];冯娜[2];韩伟[1]

机构：[1]华东理工大学药学院制药工程与过程化学教育部工程研究中心,上海市新药设计重点实验室,上海200237;[2]上海市农业科学院食用菌研究所,国家食用菌工程技术研究中心,农业部南方食用菌资源利用重点实验室,上海市农业遗传育种重点开放实验室,上海201403

年份：2020

卷号：39

期号：8

起止页码：1551

中文期刊名：菌物学报

外文期刊名：Mycosystema

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：上海市科技兴农重点攻关项目[沪农科创字(2018)第1-1号]。

语种：中文

中文关键词：灵芝;两阶段发酵;三萜类;核磁归属;增殖抑制

外文关键词：Ganoderma lingzhi;two-stage fermentation;triterpenes;NMR attribution;proliferation inhibition

摘要：通过液体振荡-静置两阶段发酵获得灵芝菌丝体,并采用硅胶柱色谱层析、反相柱层析和甲醇重结晶的方法,从中分离得到4个三萜类化合物。根据NMR、MS等波谱数据分析,化合物分别被鉴定为lanosta-7,9(11),24-trien-3α-acetoxy-26-oicacid(1)、灵芝酸R(2)、灵芝酸T(3)和灵芝酸S(4),其中化合物1的核磁信号全归属为首次报道。4个三萜类化合物均具有较好的抑制肿瘤细胞L1210及K562增殖的活性,且化合物1的体外抗肿瘤活性为首次证实,其对肿瘤细胞L1210及K562增殖的半数抑制浓度IC50分别为22.17μmol/L和54.79μmol/L。
Ganoderma lingzhi mycelia were obtained by liquid shaking-static two-stage fermentation method,and four triterpenes were isolated from the mycelia through silica gel column chromatography,reversed-phase column chromatography and methanol recrystallization.According to the analysis of spectral data of NMR and MS,these compounds were identified as lanosta-7,9(11),24-trien-3α-acetoxy-26-oic acid(1),ganoderic acid R(2),ganoderic acid T(3)and ganoderic acid S(4).The nuclear magnetic signals of compound 1 were reported for the first time.All of the four triterpenes showed good inhibitory activity on the proliferation of tumor cells L1210 and K562,and the in vitro antitumor activity of compound 1 was confirmed for the first time.IC50 values of compound 1 against tumor cells L1210 and K562 were 22.17μmol/L and 54.79μmol/L,respectively.