文献类型：专著

英文题名：Lighting Up Live-Cell and In Vivo Central Carbon Metabolism with Genetically Encoded Fluorescent Sensors

作者：Zhang, Zhuo[1,2];Cheng, Xiawei[1,2];Zhao, Yuzheng[1,2];Yang, Yi[1,3]

机构：[1]East China Univ Sci & Technol, Chinese Acad Med Sci, Shanghai Collaborat Innovat Ctr Biomfg Technol, Optogenet & Synthet Biol Interdisciplinary Res Ct, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Brain Sci, Shanghai 200031, Peoples R China

出版社：ANNUAL REVIEWS

出版日期：20200101

出版地：4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0897 USA

页数：22

纸书定价：0

语种：英文

外文关键词：central carbon metabolism; genetically encoded fluorescent sensors; real-time monitoring; fluorescence imaging

摘要：As the core component of cell metabolism, central carbon metabolism, consisting of glycolysis, the pentose phosphate pathway, and the tricarboxylic acid cycle converts nutrients into metabolic precursors for biomass and energy to sustain the life of virtually all extant species. The metabolite levels or distributions in central carbon metabolism often change dynamically with cell fates, development, and disease progression. However, traditional biochemical methods require cell lysis, making it challenging to obtain spatio-temporal information about metabolites in living cells and in vivo. Genetically encoded fluorescent sensors allow the rapid, sensitive, specific, and real-time readout of metabolite dynamics in living organisms, thereby offering the potential to fill the gap in current techniques. In this review, we introduce recent progress made in the development of genetically encoded fluorescent sensors for central carbon metabolism and discuss their advantages, disadvantages, and applications. Moreover, several future directions of metabolite sensors are also proposed.