文献类型：期刊文献

英文题名：Effective length calibration method for processing the fluorescence signal detected by charge-coupled device in capillary electrophoresis

作者：Ni, Yi[1];Liu, Chenchen[1];Chen, Jin[1];Chen, Qinmiao[1];Zhu, Xifang[2];Dou, Xiaoming[1,2]

机构：[1]East China Univ Sci & Technol, Grad Sch Sci, Inst Photon & Biomed, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]ChangZhou Inst Technol, Sch Optoelect Engn, Changzhou, Jiangsu, Peoples R China

年份：2017

卷号：40

期号：9

起止页码：2054

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20171403532715);WOS:【SCI-EXPANDED(收录号:WOS:000400943200023)】；

基金：This research was supported by the National Natural Science Foundation of China (grant number 21305089).

语种：英文

外文关键词：capillary electrophoresis; charge-coupled device; effective-length-calibration; fluorescence detection

摘要：A novel method named effective length calibration method has been developed to process the fluorescence signal detected by charge-coupled device during capillary electrophoresis. The new method treated each pixel as an individual point detector, and effectively binned a large number of pixels into a final electropherogram without losing the narrow detection window defined by a single pixel. Capillary electrophoresis separations of DNA were carried out and detected by charge-coupled device and conventional detector (photomultiplier tube). Detection properties including signalto- noise ratio, peak width, detection frequency, and tilt of detector were investigated. It was found that the new method achieved much higher signal-to-noise ratio and smaller peak width than the conventional detector did. A Detection width of 0.5 mu m was easily achieved.