文献类型：期刊文献

英文题名：Molecular cloning of an insertion sequence-like element from Vibrio anguillarum and its functional identification in E. coli

作者：Liang, Na[1]; Wu, Haizhen[1]; Ye, Jiang[1]; Xu, Peng[1]; Zhang, Yuanxing[1]; Zhang, Huizhan[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China

年份：2007

卷号：29

期号：12

起止页码：1951

外文期刊名：Biotechnology Letters

收录：EI(收录号：20074510910923)

语种：英文

外文关键词：Cloning - Enzymes - Escherichia coli - Gene expression

摘要：The tdh (thermostable direct hemolysin) gene occurs in some strains of Vibrio species. All tdh genes are flanked by insertion sequence-like elements (ISV). All previous attempts have failed to detect transposition of these ISVs. In this work, we have built a transposition detection system in E. coli and succeeded in detecting the transposition of an insertion sequence-like element at a frequency of Kmr mutants of 7.2 × 10-6. A specific flanking sequence (5′-Py-Pu-3′) was found on either side of the target duplication. ? 2007 Springer Science+Business Media B.V.