文献类型：期刊文献

英文题名：The secreted fructose 1,6-bisphosphate aldolase as a broad spectrum vaccine candidate against pathogenic bacteria in aquaculture

作者：Sun, Zhongyang[1];Shen, Binbing[1];Wu, Haizhen[1];Zhou, Xiangyu[1];Wang, Qiyao[1,3];Xiao, Jingfan[1,3];Zhang, Yuanxing[1,2,3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China;[3]Shanghai Engn Res Ctr Mariculture Anim Vaccines, Shanghai 200237, Peoples R China

年份：2015

卷号：46

期号：2

起止页码：638

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000363071200053)】；

基金：This work was financially supported by the National Natural Science Funds of China (31372550), National High Technology Research and Development Program of China (2013AA093101), Project of Marine Public Welfare Profession (201405003-2) and Shanghai Rising-Star Program (No. 13QA1401000).

语种：英文

外文关键词：FBA; Locations; Cross-protection; Aquatic pathogenic bacteria; Broad spectrum vaccine

摘要：The development of aquaculture has been hampered by different aquatic pathogens that can cause edwardsiellosis, vibriosis, or other diseases. Therefore, developing a broad spectrum vaccine against different fish diseases is necessary. In this study, fructose 1,6-bisphosphate aldolase (FBA), a conserved enzyme in the glycolytic pathway, was demonstrated to be located in the non-cytoplasmic components of five aquatic pathogenic bacteria and exhibited remarkable protection and cross-protection against these pathogens in turbot and zebrafish. Further analysis revealed that sera sampled from vaccinated turbot had a high level of specific antibody and bactericidal activity against these pathogens. Meanwhile, the increased expressions of immune response-related genes associated with antigen recognition and presentation indicated that the adaptive immune response was effectively aroused. Taken together, our results suggest that FBA can be utilized as a broad-spectrum vaccine against various pathogenic bacteria of aquaculture in the future. (C) 2015 Elsevier Ltd. All rights reserved.