文献类型：期刊文献

英文题名：Caspase-mediated LPS sensing and pyroptosis signaling in Hydra

作者：Chen, Shouwen[1];Li, Shuxin[1];Chen, Hao[1];Gong, Yuxin[1];Yang, Dahai[1,2];Zhang, Yuanxing[1,3];Liu, Qin[1]

机构：[1]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China;[3]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China

年份：2023

卷号：9

期号：29

外文期刊名：SCIENCE ADVANCES

收录：;EI(收录号：20233814754152);WOS:【SCI-EXPANDED(收录号:WOS:001037141300019)】；

基金：This work was supported by the Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism grant 2021 Sci & amp; Tech 03-28 (Shanghai Municipal Education Commission), the National Natural Science Foundation of China (nos. 32025038 and 32002429), the China Postdoctoral Science Foundation (no. 2020 M671033), and the ECUST-OPM Open Fund (no. 20220701).& nbsp;& nbsp;

语种：英文

外文关键词：Network security

摘要：Inflammatory caspases sensing lipopolysaccharide (LPS) to drive gasdermin (GSDM)-mediated pyroptosis is an important immune response mechanism for anti-infection defense in mammals. In this work, we resolved an LPS-induced and GSDM-gated pyroptosis signaling cascade in Cnidarians. Initially, we identified a functional GSDM protein, HyGSDME, in Hydra, executing cytosolic LPS-induced pyroptosis in a caspase-dependent manner. Further, we identified a proinflammatory caspase, HyCaspA, capable of sensing cytosolic LPS by an uncharacterized N-terminal domain relying on its unique hydrophobic property, thereby triggering its oligomerization and self-activation. Subsequently, the LPS-activated HyCaspA cleaved an apoptotic caspase, HyCARD2, to trigger HyGSDME-gated pyroptosis. Last, HyGSDME exhibited an enriched distribution on the ectodermal layer of Hydra polyps, exerting a canonical immune defense function against surface-invading bacteria. Collectively, our work resolved an ancient pyroptosis signaling cascade in Hydra, suggesting that inflammatory caspases sensing cytosolic LPS to initiate GSDM-gated pyroptosis are a conserved immune defense mechanism from Cnidarians to mammals.