文献类型：期刊文献

英文题名：Selective bioimaging of cancer cells and detection of HSA with indomethacin-based fluorescent probes

作者：Chen, Cong[1];Yao, Yuhua[1];Wang, Weisi[2];Duan, Liping[2];Zhang, Weibing[1];Qian, Junhong[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Chinese Ctr Dis Control & Prevent, Natl Inst Parasit Dis, Shanghai 200032, Peoples R China

年份：2020

卷号：241

外文期刊名：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

收录：;EI(收录号：20202808923753);WOS:【SCI-EXPANDED(收录号:WOS:000570309900006)】；

基金：This work was financially supported by NSFC (21576085 and 21974045).

语种：英文

外文关键词：Fluorescent probe; Indomethacin; Cancer cellular imaging; Human serum albumin; Molecular docking

摘要：Two fluorescent probes were designed by connecting indomethacin to coumarin through different linkers. The introduction of indomethacin quenched the fluorescence of coumarin-based probes with apparent red-shifts in the absorption and emission maxima, probably due to the photoinduced electron transfer (PET) from the indomethacin to the fluorophore and the formation of folding conformation. The addition of human serumalbumin (HSA) triggered about 40-fold fluorescence enhancements of ADC-IMC-2 and ADC-IMC-6 with 85nmblue-shifts. The probe with longer spacer ADC-IMC-6 exhibited ratiometric fluorescent response toward HSA, and that with shorter linker showed "off-on" fluorescence response to HSA. However, insignificant spectral changes of the reference compounds (ADC-6 and ADC-2) initiated by HSA implied that indomethacin played critical role in the identification of HSA. The competitive assays and molecular docking results reveal that the indomethacin in ADC-IMC-6 could tightly combine at drug site I of HSA. Fluorescence bio-imaging experiments show that both probes could distinguish cancer cells from normal cells. (C) 2020 Elsevier B.V. All rights reserved.