文献类型：期刊文献

英文题名：Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris

作者：Zhou, Jin[1];Chu, Ju[1];Wang, Yong-Hong[1];Wang, Hui[1];Zhuang, Ying-Ping[1];Zhang, Si-Liang[1]

机构：[1]E China Univ Sci & Technol, Natl Engn Res Ctr Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：30

期号：4

起止页码：651

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000253357800011)】；

语种：英文

外文关键词：diabetes; exendin-4; glucagon peptide analogue; Pichia pastoris

摘要：Exendin-4, a peptide analogue of glucagon-like peptide-1 (GLP-1), has been developed for treatment of type 2 diabetes. Herein, the secretive exendin-4 fusion protein, expressed by methanol induction in Pichia pastoris system, was purified to homogeneity by chromatography followed by enterokinase cleavage of the fusion protein and subsequent purification of the recombinant exendin-4. Purity of the recombinant exendin-4 was 95.6%. Bioactivity assay revealed that it had glucose-lowering and insulin-releasing action in vivo.