文献类型：期刊文献

英文题名：Metabolic Engineering of Gas-Fermenting Clostridium ljungdahlii for Efficient Co-production of Isopropanol, 3-Hydroxybutyrate, and Ethanol

作者：Jia, Dechen[1,2];He, Meiyu[1,3];Tian, Yi[1,3];Shen, Shaohuang[4];Zhu, Xianfeng[3];Wang, Yonghong[4];Zhuang, Yingping[4];Jiang, Weihong[1];Gu, Yang[1]

机构：[1]Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Shanghai Inst Plant Physiol & Ecol, Key Lab Synthet Biol,State Key Lab Plant Carbon N, Shanghai 200032, Peoples R China;[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]Henan Univ, Sch Life Sci, Key Lab Plant Stress Biol, State Key Lab Cotton Biol, Kaifeng 475004, Peoples R China;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2021

卷号：10

期号：10

起止页码：2628

外文期刊名：ACS SYNTHETIC BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000709769300020)】；

基金：This work was supported by the National Key R&D Program of China (2018YFA0901500), the National Natural Science Foundation of China (31921006, 31630003), the DNL Cooperation Fund, CAS (DNL202013), Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project (TSBICIP-KJGG-016), and the Science and Technology Commission of Shanghai Municipality.

语种：英文

外文关键词：Clostridium ljungdahlii; co-production; isopropanol; 3-hydroxybutyrate; ethanol; C1 gases

摘要：Rational design and modification of autotrophic bacteria to efficiently produce high-value chemicals and biofuels are crucial for establishing a sustainable and economically viable process for one-carbon (C1) source utilization, which, however, remains a challenge in metabolic engineering. In this study, autotrophic Clostridium ljungdahlii was metabolically engineered to efficiently co-produce three important bulk chemicals, isopropanol, 3-hydroxybutyrate (3-HB), and ethanol (together, IHE), using syngas (CO2/CO). An artificial isopropanol-producing pathway was first constructed and optimized in C. ljungdahlii to achieve an efficient production of isopropanol and an unexpected product, 3-HB. Based on this finding, an endogenous active dehydrogenase capable of converting acetoacetate to 3-FIB was identified in C. ljungdahlii, thereby revealing an efficient 3-HB-producing pathway. The engineered strain was further optimized to reassimilate acetic acid and synthesize 3-HB by introducing heterologous functional genes. Finally, the best-performing strain was able to produce 13.4, 3.0, and 28.4 g/L of isopropanol, 3-HB, and ethanol, respectively, in continuous gas fermentation. Therefore, this work represents remarkable progress in microbial production of bulk chemicals using C1 gases.