文献类型：期刊文献

英文题名：Facilitated vascularization and enhanced bone regeneration by manipulation hierarchical pore structure of scaffolds

作者：Liu, Yang[1,2];Yang, Shengbing[4];Cao, Lingyan[5];Zhang, Xiaohui[1,2];Wang, Jing[1,2];Liu, Changsheng[2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[4]Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Shanghai Key Lab Orthopaed Implants, Dept Orthopaed Surg,Sch Med, Shanghai 200011, Peoples R China;[5]Shanghai Jiao Tong Univ, Peoples Hosp 9, Oral Bioengn & Regenerat Med Lab, Sch Med,Dept Prosthodont,Shanghai Key Lab Stomato, Shanghai 200011, Peoples R China

年份：2020

卷号：110

外文期刊名：MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

收录：;EI(收录号：20200308031521);WOS:【SCI-EXPANDED(收录号:WOS:000527395900011)】；

基金：The authors wish to express their gratitude to the financial supports from the Innovative Research Group Project of the National Natural Science Foundation of China (No. 51621002), National Natural Science Foundation of China (31870953), and National Key Research and Development Program of China (2018YFE0201500). This study is also supported by 111 Project (B14018).

语种：英文

外文关键词：Scaffold; Hierarchical structure; Vascularization; Bone regeneration

摘要：Sufficient vascularization is quite important for preventing cell death and promoting host integration during the repair of the critical sized bone defects. Porous structure providing enough space for the ingrowth of vessels is an essential consideration during the scaffold's development. In this study, we designed and fabricated three kinds of porous structured scaffolds based on poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx), such as mono-structured PHBHHx scaffolds with macro pores (PH-1), di-structured PHBHHx scaffolds with macro-meso pores (PHS-2), and tri-structured PHBHHx scaffolds with macro-micro-meso pores (PHS-3), respectively. In vitro effects of the hierarchical porous scaffolds on human umbilical vein endothelial cells (HUVECs), such as cell attachment, glucose and lactate detection, relative gene expressions of endothelial markers were investigated. The PHS-3 scaffolds exhibited preferential potency of inducing better angiogenesis in vitro. Consequently, the hierarchical porous scaffolds were applied to load rhBMP-2 and repair the critical sized bone defect (15 mm) in rabbits. Microangiography analysis by three dimensional micro-computed tomographic (micro-CT) demonstrated that the volume of blood vessels within the defect area was higher in the rhBMP-2 loaded PHS-3 (PHS-3/rhBMP-2) than that in other rhBMP-2 loaded porous scaffolds with simplex or double scaled pores (PH-1/rhBMP2 or PHS-2/rhBMP-2) at 4 weeks and 8 weeks, which implied that multi-level porous structure was conducive to nutrition transmission and revascularization. Further investigations of orthotopic bone formation by micro-CT, histological and immunohistochemistry analysis confirmed the most accelerated new bone formation rate in the PHS-3/rhBMP-2 group. The maximum load value of the regenerated bone induced by PHS-3/rhBMP-2 at 12 weeks was 258.47 +/- 14.77 N which did not show significant difference from the normal bone of 268.81 +/- 12.05 N. These results highlighted that introducing multi-level pores into the biocompatible scaffolds may be an effective approach to promote angiogenesis and bone regeneration.