文献类型：期刊文献

英文题名：Tumor Microenvironment-Responsive Hydrogel for Direct Extracellular ATP Imaging-Guided Surgical Resection with Clear Boundaries

作者：Yang, Jingyi[1];Qu, Jiahao[1];Teng, Xuanming[1];Zhu, Weiping[1,2];Xu, Yufang[1];Yang, Yangyang[1];Qian, Xuhong[1,3]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2023

卷号：12

期号：28

外文期刊名：ADVANCED HEALTHCARE MATERIALS

收录：;EI(收录号：20232314181656);WOS:【SCI-EXPANDED(收录号:WOS:000999198000001)】；

基金：This work was supported by the National Natural Science Foundation of China (grant no. 82161148008), the Research Funds of Happiness Flower ECNU (2020JK2103), Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism and Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：DNA hydrogels; extracellular ATP imaging; fluorescence-guided surgery resection

摘要：Most solid tumors are clinically treated using surgical resection, and the presence of residual tumor tissues at the surgical margins often determines tumor survival and recurrence. Herein, a hydrogel (Apt-HEX/Cp-BHQ1 Gel, termed AHB Gel) is developed for fluorescence-guided surgical resection. AHB Gel is constructed by tethering a polyacrylamide hydrogel and ATP-responsive aptamers together. It exhibits strong fluorescence under high ATP concentrations corresponding to the TME (100-500 mu m) but shows little fluorescence at low ATP concentrations (10-100 nm) such as those in normal tissues. AHB Gel can rapidly (within 3 min) emit fluorescence after exposure to ATP, and the fluorescence signal only occurs at sites exposed to high ATP, resulting in a clear boundary between the ATP-high and ATP-low regions. In vivo, AHB Gel exhibits specific tumor-targeting capacity with no fluorescence response in normal tissue, providing clear tumor boundaries. In addition, AHB Gel has good storage stability, which is conducive to its future clinical application. In summary, AHB Gel is a novel tumor microenvironment-targeted DNA-hybrid hydrogel for ATP-based fluorescence imaging. It can enable the precise imaging of tumor tissues, showing promising application in fluorescence-guided surgeries in the future.