文献类型：期刊文献

英文题名：The transcription factor ACE3 controls cellulase activities and lactose metabolism via two additional regulators in the fungus Trichoderma reesei

作者：Zhang, Jiajia[1];Chen, Yumeng[1];Wu, Chuan[1];Liu, Pei[1];Wang, Wei[1];Wei, Dongzhi[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2019

卷号：294

期号：48

起止页码：18435

外文期刊名：JOURNAL OF BIOLOGICAL CHEMISTRY

收录：;EI(收录号：20195107837930);WOS:【SCI-EXPANDED(收录号:WOS:000505547900036)】；

基金：This work was supported by the Fundamental Research Funds for the Central Universities (Grant 222201714053) and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering (Grant 2018). The authors declare that they have no conflicts of interest with the contents of this article.

语种：英文

外文关键词：Trichoderma reesei; transcription factor; protein-protein interaction; cellulase; DNA binding protein; protein-DNA interaction; gene expression; fungi; Hypocrea jecorina; gene knockout; ace3; protein-protein interaction; transcription factor; Trichoderma reesei (Hypocrea jecorina); Zn(II)2Cys6 protein; renewables; gene regulation; hydrolase; transcriptomics

摘要：Fungi of the genus Trichoderma are a rich source of enzymes, such as cellulases and hemicellulases, that can degrade lignocellulosic biomass and are therefore of interest for biotechnological approaches seeking to optimize biofuel production. The essential transcription factor ACE3 is involved in cellulase production in Trichoderma reesei; however, the mechanism by which ACE3 regulates cellulase activities is unknown. Here, we discovered that the nominal ace3 sequence in the T. reesei genome available through the Joint Genome Institute is erroneously annotated. Moreover, we identified the complete ace3 sequence, the ACE3 Zn(II)(2)Cys(6) domain, and the ACE3 DNA-binding sites containing a 5?-CGGAN(T/A)(3)-3? consensus. We found that in addition to its essential role in cellulase production, ace3 is required for lactose assimilation and metabolism in T. reesei. Transcriptional profiling with RNA-Seq revealed that ace3 deletion down-regulates not only the bulk of the major cellulase, hemicellulase, and related transcription factor genes, but also reduces the expression of lactose metabolism?related genes. Additionally, we demonstrate that ACE3 binds the promoters of many cellulase genes, the cellulose response transporter gene crt1, and transcription factor?encoding genes, including xyr1. We also observed that XYR1 dimerizes to facilitate cellulase production and that ACE3 interacts with XYR1. Together, these findings uncover how two essential transcriptional activators mediate cellulase gene expression in T. reesei. On the basis of these observations, we propose a model of how the interactions between ACE3, Crt1, and XYR1 control cellulase expression and lactose metabolism in T. reesei.