文献类型：期刊文献

英文题名：Efficient production of (S)-naproxen with (R)-substrate recycling using an overexpressed carboxylesterase BsE-NP01

作者：Liu, Xiang[1]; Xu, Jian-He[1]; Pan, Jiang[1]; Zhao, Jing[1]

机构：[1] Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

年份：2010

卷号：162

期号：6

起止页码：1574

外文期刊名：Applied Biochemistry and Biotechnology

收录：EI(收录号：20104913458280)

语种：英文

外文关键词：Escherichia coli - Hydrolysis - Bacteriology - Enantiomers - Enzymes - Cloning - Enantioselectivity - Esters

摘要：An (S)-enantioselective esterase from Bacillus subtilis ECU0554, named BsE-NP01, has been cloned and over-expressed in a heterologous host Escherichia coli BL21. BsE-NP01 was shown to be a carboxylesterase with a molecular mass of about 32 kDa, and temperature and pH optima at 50 °C and 8.5, respectively. It could catalyze the selective hydrolysis of the (S)-enantiomer of racemic naproxen methyl ester, giving optically pure (S)-naproxen with 98% enantiomeric excess. A mechanic-grinding approach to substrate dispersion was also reported, which was considered to be an alternative to take the place of deleterious surfactants such as Tween-80, with improved performance of the hydrolysis reaction. Batch production of (S)-naproxen was repeatedly carried out in a solid-water biphasic system at 2-L scale, achieving an average total yield of about 85% after ten runs with complete recycling of (R)-substrate. [Figure not available: see fulltext.] ? 2010 Springer Science+Business Media, LLC.