文献类型：期刊文献

英文题名：Statistical optimization of medium for the production of pyruvate oxidase by the recombinant Escherichia coli

作者：Zhao, Jie[1];Wang, Yonghong[1];Chu, Ju[1];Zhang, Siliang[1];Zhuang, Yingping[1];Yuan, Zhongyi[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：35

期号：4

起止页码：257

外文期刊名：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000254249600008)】；

语种：英文

外文关键词：pyruvate oxidase; recombinant E coli; medium optimization; response surface methodology

摘要：Pyruvate oxidase (PyOD) is a very useful enzyme for clinical diagnostic applications and environmental monitor. Optimization of the fermentation medium for maximization of PyOD constitutively, production by Escherichia coli DH5 alpha/pSMLPyOD was carried out. Response surface methodology (RSM) was used to optimize the medium constituents. A 2(6-2) fractional factorial design (first order model) was carried out to identify the significant effect of medium components towards PyOD production. Statistical analysis of results shows that yeast extract, ammonium sulfate and composite phosphate were significant factors on PyOD production. The optimized values of these three factors were obtained by RSM based on the result of a 2(3) central composite rotatable design. Under these proposed optimized medium, the model predicted a PyOD activity of 610 U/L and via experimental rechecking the model, an activity of 670 U/L was attained.