文献类型：期刊文献

英文题名：Proteins Separation and Purification by Expanded Bed Adsorption and Simulated Moving Bed Technology

作者：Li, Ping[1]; Gomes, Pedro Ferreira[2]; Loureiro, José M.[2]; Rodrigues, Alirio E.[2]

机构：[1] East China University of Science and Technology, State Key Laboratory of Chemical Engineering, College of Chemical Engineering, 130 Meilong Road, Xuhui, Shanghai, 200237, China; [2] University of Porto, Faculty of Engineering, Laboratory of Separation and Reaction Engineering [LSRE], Rua Dr. Roberto Frias, s/n, Porto, 4200-465, Portugal

年份：2015

起止页码：1

外文期刊名：Continuous Processing in Pharmaceutical Manufacturing

收录：EI(收录号：20182705467445)

语种：英文

外文关键词：Purification - Ions - Chromatography - Mammals - Proteins

摘要：The development of techniques and methods for proteins separation and purification has been an essential prerequisite for many of the advancements made in biotechnology. Most separation and purification protocols require more than one step to achieve the desired level of protein purity. Usually, a three-step separation and purification strategy is presented, which includes capture, intermediate separation and purification, and final polishing during a downstream protein separation and purification process. This chapter describes the developments made at the Laboratory of Separation and Reaction Engineering (LSRE) for proteins separation and purification by expanded bed chromatography and salt gradient ion exchange simulated moving bed (SMB) technology. Experiments were carried out for bovine serum albumin (BSA) and myoglobin adsorption/desorption in expanded beds. Some strategies are also discussed for the selection of salt gradient and the selection of flow rate in each section of salt gradient ion exchange SMB. ? 2015 Wiley-VCH Verlag GmbH & Co. KGaA. All rights reserved.