文献类型：期刊文献

英文题名：A light-dark shift strategy derived from light-responded metabolic behaviors for polyketides production in marine fungus Halorosellinia sp.

作者：Zhang, Xiaoxu[1];He, Hao[1];Yin, Ying[1];Zhou, Weiqiang[1];Cai, Menghao[1];Zhou, Xiangshan[1];Zhang, Yuanxing[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：221

起止页码：34

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20160501875777);WOS:【SCI-EXPANDED(收录号:WOS:000371459300005)】；

基金：This work was financially supported by the Ministry of National Science and Technology Create Significant New Drugs (2012ZX09102101), the Fundamental Research Funds for the Central Universities (22A201514040) and Chinese National High Technology Research and Development Program (2012AA092105). We thank Prof. Zhigang She, Sun Yat-Sen University for supply of the strain and Dr. Ke Na, Shanghai Institute of Pharmaceutical Industry, for supplying the 1403C and 1403R standards.

语种：英文

外文关键词：Fermentation; Light regulation; Light-dark shift; Anticancer polyketide; Halorosellinia sp.; Marine fungus

摘要：Light, as an important environmental signal, generally brings about a broad regulation in fungal metabolism. In this work, we aim to explore the light-responded metabolic rules so as to further develop a feasible and effective light regulation strategy for production of anticancer polyketide 1403C by marine fungus Halorosellinia sp.. Light derived production enhancement of polyketides was first found in shake flask. To further understand this well working black box, light-responded cell growth, polyketides biosynthesis, metabolic behaviors (enzymes activities and organic acids levels) and mycelia morphology were then investigated in 5-L bioreactor. By comparing cultures under constant irradiation and dark conditions, the entire bioprocess was divided into two phases. During 0-60 h, light presumably stimulated relevant metabolism to generate sufficient energy, NADPH and carbon skeleton, particularly malonyl-CoA, which was favorable for mycelia growth and polyketides accumulation. After 60 h, light did harm to biomass and polyketides production. Consequently, a light-dark shift strategy was proposed and verified in 5-L bioreactor. It led to a maximal 1403C production of 1.67 g/L, which was 24% and 74% higher than those obtained under constant irradiation and dark conditions, respectively. (C) 2016 Elsevier B.V. All rights reserved.