文献类型：期刊文献

英文题名：Reconstruction and analysis of a genome-scale metabolic model of the vitamin C producing industrial strain Ketogulonicigenium vulgare WSH-001

作者：Zou, Wei[1,3]; Liu, Liming[1,2,3]; Zhang, Jing[4]; Yang, Haoru[1,3]; Zhou, Maoda[1,3]; Hua, Qiang[2]; Chen, Jian[1,3]

机构：[1] State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; [2] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [3] The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; [4] College of Bioengineering, Sichuan University of Science and Engineering, 180 Huixing Road, Zigong, Sichuan 643000, China

年份：2012

卷号：161

期号：1

起止页码：42

外文期刊名：Journal of Biotechnology

收录：EI(收录号：20123015281325)

语种：英文

外文关键词：Physiological models - Genes - Sulfur compounds - Physiology - Amino acids - Production efficiency

摘要：Ketogulonicigenium vulgare WSH001 is an industrial strain commonly used in the vitamin C producing industry. In order to acquire a comprehensive understanding of its physiological characteristics, a genome-scale metabolic model of K. vulgare WSH001, iWZ663, including 830 reactions, 649 metabolites, and 663 genes, was reconstructed by genome annotation and literature mining. This model was capable of predicting quantitatively the growth of K. vulgare under l-sorbose fermentation conditions and the results agreed well with experimental data. Furthermore, phenotypic features, such as the defect in sulfate metabolism hampering the syntheses of l-cysteine, l-methionine, coenzyme A (CoA), and glutathione, were investigated and provided an explanation for the poor growth of K. vulgare in monoculture. The model presented here provides a validated platform that can be used to understand and manipulate the phenotype of K. vulgare to further improve 2-KLG production efficiency. ? 2012 Elsevier B.V.