文献类型：期刊文献

英文题名：Lactobacillus rhamnosus GG triggers intestinal epithelium injury in zebrafish revealing host dependent beneficial effects

作者：Zhang, Zhen[1,2];Zhang, Hong-Ling[3];Yang, Da-Hai[4];Hao, Qiang[3];Yang, Hong-Wei[3];Meng, De-Long[3];de Vos, Willem Meindert[5,6];Guan, Le-Luo[2];Liu, Shu-Bin[3];Teame, Tsegay[3,7];Gao, Chen-Chen[3];Ran, Chao[1];Yang, Ya-Lin[1];Yao, Yuan-Yuan[1];Ding, Qian-Wen[1];Zhou, Zhi-Gang[3]

机构：[1]Chinese Acad Agr Sci, Inst Feed Res, Key Lab Feed Biotechnol, Minist Agr & Rural Affairs, Beijing, Peoples R China;[2]Univ British Columbia, Fac Land & Food Syst, Vancouver, BC, Canada;[3]Chinese Acad Agr Sci, Inst Feed Res, China Norway Joint Lab Fish Gut Microbiota, Beijing, Peoples R China;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[5]Wageningen Univ & Res, Lab Microbiol, Wageningen, Netherlands;[6]Univ Helsinki, Fac Med, Human Microbiome Res Program, Helsinki, Finland;[7]Tigray Agr Res Inst, Mekelle, Ethiopia

年份：2024

卷号：3

期号：2

外文期刊名：IMETA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001190415100001)】；

基金：This work was supported by the National Natural Science Foundation of China (NSFC 31925038, 32172991, 32330110), China Scholarship Council (CSC) under the State Scholarship Fund (CSC No. 202203250030) and Agriculture Science and Technology Innovation Progam (ASTIP) of the Chinese Academy of Agricultural Sciences (CAAS-ZDRW202305). We thank Mrs Wu Yao from the State Key Laboratory of Plant Genomics (SKLPG) at the Institute of Microbiology of Chinese Academy of Sciences for the help in the MST experiment.

语种：英文

外文关键词：intestinal mucosal damage; Lactobacillus rhamnosus GG; probiotic safety; SpaC; zebrafish

摘要：Lactobacillus rhamnosus GG (LGG), the well-characterized human-derived probiotic strain, possesses excellent properties in the maintenance of intestinal homeostasis, immunoregulation and defense against gastrointestinal pathogens in mammals. Here, we demonstrate that the SpaC pilin of LGG causes intestinal epithelium injury by inducing cell pyroptosis and gut microbial dysbiosis in zebrafish. Dietary SpaC activates Caspase-3-GSDMEa pathways in the intestinal epithelium, promotes intestinal pyroptosis and increases lipopolysaccharide (LPS)-producing gut microbes in zebrafish. The increased LPS subsequently activates Gaspy2-GSDMEb pyroptosis pathway. Further analysis reveals the Caspase-3-GSDMEa pyroptosis is initiated by the species-specific recognition of SpaC by TLR4ba, which accounts for the species-specificity of the SpaC-inducing intestinal pyroptosis in zebrafish. The observed pyroptosis-driven gut injury and microbial dysbiosis by LGG in zebrafish suggest that host-specific beneficial/harmful mechanisms are critical safety issues when applying probiotics derived from other host species and need more attention.