文献类型：期刊文献

英文题名：Photolarvicidal effect of thienyl 1,3,4-thia(oxa)diazoles and their potential DNA photocleavage

作者：Huang, QC; Liu, LG; Xiao, GY; Xu, YF; Qian, X

机构：[1]E China Univ Sci & Technol, Inst Pesticides & Pharmaceut, Shanghai 200237, Peoples R China;[2]Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China;[3]E China Univ Sci & Technol, Natl Engn Res Ctr Biotechnol, Shanghai 200237, Peoples R China

年份：2004

卷号：79

期号：2

起止页码：42

外文期刊名：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000221888600003)】；

语种：英文

外文关键词：thienyl 1,3,4-thia(oxa)diazoles; photoactivated larvicidal activity; DNA photocleavage

摘要：The photolarvicidal activities and DNA photocleavage effects of 10 thienyl 1,3,4-thia(oxa)diazoles were investigated. The compounds, as novel photosensitizers, showed strong larvicidal activities against Pseudaletia separata only under irradiated conditions. Thiadiazole containing Br atom (I-2) exhibited the highest photolarvicidal activity with mortality about 70.7%. The degree of mortality was increased with increasing accessibility period of the insects to photosensitizer-loaded leaves during exposure to light. The compounds also exhibited considerable DNA photocleavage activities on agarose gel electrophoresis that scissored supercoiled pBR322 DNA into two bands, scDNA and ocDNA. Oxadiazole containing Br atom (II2) nicked pBR322 DNA to produce ocDNA with highest conversion about 76.21%. DTT decreased DNA photocleavage effects, but SOD increased photoactivities, in vitro. Br was important in improving the biofunctioning of the photosensitizers. These results demonstrate the potential mode of action of thienyl-thia(oxa)diazole photosensitizers as insecticidal agents. (C) 2004 Elsevier Inc. All rights reserved.