文献类型：期刊文献

中文题名：Programmed co-assembly of DNA-peptide hybrid microdroplets by phase separation

作者：Shengtao Yao[1];Yue Liao[1];Rizhao Pan[1];Weiping Zhu[1];Yufang Xu[1];Yangyang Yang[1];Xuhong Qian[1,2]

机构：[1]School of Pharmacy,Shanghai Key Laboratory of Chemical Biology,East China University of Science and Technology,Shanghai 200237,China;[2]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology Shanghai 200237,China

年份：2022

卷号：33

期号：3

起止页码：1545

中文期刊名：Chinese Chemical Letters

外文期刊名：中国化学快报（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2021_2022】；PubMed;

基金：supported by the National Natural Science Foundation of China (No. 31600802)。

语种：英文

中文关键词：Self-assembly;Microdroplet;DNA origami;Phase separation;Elastin-like polypeptides

摘要：Biopolymers, including DNA and peptides have been used as excellent self-assembling building blocks for programmable single-component or hybrid materials, due to their controlled molecular interactions.However, combining two assembling principles of DNA-based programmability and peptide-based specific molecular interactions for hybrid structures to microscale has not yet been achieved. In this study,we describe a hybrid microsystem that emerges from the co-assembly of DNA origami structure and short elastin-like polypeptide conjugated oligonucleotides, and initiates liquid-liquid phase separation to generate microdroplets upon heating above the transition temperature. Moreover, the hybrid microdroplets are capable for guest molecule trapping and perform bi-/tri-enzymatic cascades with rate enhancements as open “microreactors”. Our programmed assembled DNA-peptide microsystem represents a new combination of DNA nanotechnology and peptide science and opens potential application routes toward lifeinspired biomaterials.