文献类型：期刊文献

中文题名：Scale-down反应器中的周期性溶氧波动对谷氨酸发酵的影响

英文题名：Effect of Periodic Dissolved Oxygen Fluctuations on Glutamate Fermentation in Scale-down Reactor

作者：张静[1];夏建业[1];储炬[1];庄英萍[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,国家生化工程技术研究中心,上海200237

年份：2012

卷号：38

期号：4

起止页码：477

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：Scale—down反应器;溶氧梯度;谷氨酸发酵

外文关键词：scale-down reactor; DO gradient; L-glutamate fermentation

摘要：应用单室Scale-down反应器系统模拟大规模反应器内的溶氧(DO)浓度梯度,通过控制搅拌桨转速使DO水平(波动范围0～30%,波动周期2、4、6 h)产生矩形波动,从而研究溶氧波动条件对谷氨酸发酵的影响,尤其是菌体生长及产物合成等的变化。与DO水平30%的对照实验进行比较后发现:DO周期性波动条件下,谷氨酸棒杆菌更倾向于利用葡萄糖产生菌体而非积累产物谷氨酸。与较长波动周期(4 h或6 h)相比,短波动周期(2 h)时最终菌体浓度升高(10.03 g/L),最大比生长速率降低(0.112 h^(-1)),产物谷氨酸及副产物乳酸浓度降低(分别为23 g/L和10.5 g/L)。
A laboratory scale one-compartment scale-down system was used to simulate dissolved oxygen （DO） gradients occurred in large-scale. Rectangular DO oscillations were established by manipulating the rotation speed of impeller,at oscillations periods of 2 h,4 h and 6 h and DO ranging from 0 to 30%. The effect of oscillating DO on the glutamate fermentation process was investigated,particularly on the cell growth and productivity of the glutamate or lactate. Cultures carried out at constant DO 30 ~ of air saturation were used for comparison. It was shown that Corynebacterium glutarnicurn preferred to use glucose for growth but not for product accumulation under oscillating DO condition. Oscillations at the shortest period tested （i. e. ,2 h） resulted in higher final biomass concentration （10.03 g/L） and lower maximum specific growth rate（0. 112 h 1）,glutamate production（23 g/L） and lactate accumulation（10.5 g/L） compared with cultures carried out at longer oscillations periods （4 h and 6 h）.