文献类型：期刊文献

英文题名：Selective visualization of live-cell mitochondrial thiophenols and their induced oxidative stress process by a rationally designed rhodol-based fluorescent probe

作者：Liu, Qimeng[1]; Li, Anyang[1]; Li, Xiaokang[2]; Li, Bin[3]; Zhang, Yanhui[1]; Li, Jian[2]; Guo, Yuan[1]

机构：[1] Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, National Demonstration Center for Experimental Chemistry Education, College of Chemistry and Materials Science, Northwest University, Xi'an, 710127, China; [2] State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China; [3] Key Laboratory of Resource Biology and Biotechnology in Western China of the Ministry of Education, Northwest University, Xi'an, 710069, China

年份：2019

卷号：283

起止页码：820

外文期刊名：Sensors and Actuators, B: Chemical

收录：EI(收录号：20185206297197)

语种：英文

外文关键词：Fluorescence imaging - Oxidative stress - Lanthanum compounds - Oxygen - Fluorescence spectroscopy - Cytology - Mitochondria

摘要：Mitochondria as cellular powerhouses are the preferential targets affected by thiophenols, an important class of highly toxic environmental pollutants, and are linked to the production of pathogenic reactive oxygen species (ROS) induced by trace thiophenol residues. For real-time and accurate sensing, it is critically important to develop highly sensitive fluorescent probes for the specific detection of mitochondrial thiophenols. Herein, we report the first mitochondria-targeted fluorescent probe (ROAL) to image thiophenols in living cells. The development of ROAL was based on a novel red-emitting rhodol derivative (ROAP). ROAL proved to be highly selective to thiophenols among various analytes including aliphatic thiols, and renders an ultrasensitive off-on fluorescence response to thiophenols with a remarkable detection limit (8.1 nM). The probe efficiently stains mitochondria with a high Pearson's co-localization coefficient (0.95) using Mito Tracker Green FM as reference, thereby ensuring the specific detection of mitochondrial thiophenols in living HepG2 and HeLa cells. In particular, using this probe we for the first time proved that endogenous reactive oxygen species have the capacity to eliminate thiophenols in living cells, suggesting that thiophenols might induce cellular oxidative stress. ? 2018 Elsevier B.V.