文献类型：期刊文献

英文题名：Insight into the Cellular Internalization and Cytotoxicity of Graphene Quantum Dots

作者：Wu, Congyu[1];Wang, Chong[2];Han, Ting[1];Zhou, Xuejiao[1];Guo, Shouwu[1];Zhang, Jingyan[2]

机构：[1]Shanghai Jiao Tong Univ, Res Inst Micro Nano Sci & Technol, Minist Educ, Key Lab Thin Film & Microfabricat, Shanghai 200240, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：2

期号：12

起止页码：1613

外文期刊名：ADVANCED HEALTHCARE MATERIALS

收录：;EI(收录号：20200708176213);WOS:【SCI-EXPANDED(收录号:WOS:000328344000010)】；

基金：We thank the National Science foundation of China (Nos. 91123011, 90923041, and 31070742), the state key laboratory of bioreactor engineering (No. 2060204), 111 Project (No. B07023),the Science and Technology Commission of Shanghai Municipality (No. 12nm0503500), and National "973 Program" of China (No. 2010CB933900) for financial support of this work.

语种：英文

外文关键词：cellular internalization; cytotoxicity; distribution; graphene quantum dots

摘要：Graphene quantum dots (GQDs), owing to their unique morphology, ultra-small lateral sizes, and exceptional properties, hold great promise for many applications, especially in the biomedical field. In this work, the cellular internalization, distribution, and cytotoxicity of the GQDs are explored complementarily using transmission electron microscopy, confocal laser scanning microscopy, UV-vis, and fluorescence spectroscopies, and flow cytometry with human gastric cancer MGC-803 and breast cancer MCF-7 cells. It is demonstrated that the GQDs are internalized primarily through caveolae-mediated endocytosis. The effects of GQDs on the cell viability, internal cellular reactive oxygen species (ROS) level, mitochondrial membranes potential, and cell cycles show that the cytotoxicity of GQDs is lower than that of the micrometer-sized graphene oxide (GO). The low cytotoxicity and size consistence render GQDs appropriate for biomedical application.