文献类型：期刊文献

英文题名：Oligosaccharides with different glycosidic bonds regulate gut microbiota: differential modulation of bacterial and fungal communities and their metabolism in vitro

作者：Lu, Xiaoxuan[1,2,3];Zou, Jiaqi[1,2,3];Feng, Xiangru[1,2,3];Han, Geng[1,2,3];Zhu, Liangliang[1,2,3];Chen, Yijia[1,2,3];Yang, You[2,4];Jin, Jiayang[1,2,3];Ji, Xiaoguo[1,2,3];Zhao, Liming[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Shanghai 200237, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol SCI, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China

年份：2025

卷号：221

外文期刊名：FOOD RESEARCH INTERNATIONAL

收录：;EI(收录号：20253719161518);WOS:【SCI-EXPANDED(收录号:WOS:001584077200001)】；

基金：This research was funded by The Young Scientists Fund of the National Natural Science Foundation of China (32302102), Natural Science Foundation of Shanghai(23ZR1415400), the China Postdoctoral Science Foundation (2023M731087) and Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission).

语种：英文

外文关键词：Disaccharides; Glycosidic bonds; Gut bacterial microbiota; Gut fungal microbiota; Short-chain fatty acids (SCFAs); Microbial metabolites

摘要：Oligosaccharides significantly impact host health by modulating gut microbial composition and metabolic activities. However, the influence of oligosaccharides with different glycosidic bonds on intestinal microecology remains unclear, hindering their functional food applicability. This study examined the impact of six disaccharides, including trehalose (alpha-1,1), maltose (alpha-1,4), isomaltose (alpha-1,6), cellobiose (beta-1,4), beta-lactose (beta-1,4), and gentiobiose (beta-1,6), at concentrations of 10 mM. The results showed that alpha-disaccharides (maltose and isomaltose) were metabolized faster than beta-disaccharides (cellobiose and gentiobiose). They also significantly increased both bacterial and fungal richness (Chao1 and ACE indexes) and diversity (PD whole tree) compared to beta-disaccharides (p < 0.05). Both alpha- and beta-disaccharides significantly enriched Megamonas funiformis and Phocaeicola vulgatus while reducing Klebsiella pneumoniae abundance. Notably, beta-disaccharides enhanced Faecalibacterium prausnitzii and Paraprevotella clara proliferation. Despite a less pronounced effect on fungi than bacteria, isomaltose and gentiobiose substantially altered fungal composition by reducing Candida africana abundance and promoting Penicillium citrinum growth. These oligosaccharides promoted complex gut microbial networks, with key taxa including Occultifur kilbournensis, Ruminococcaceae bacterium, and Streptococcus salivarius. Notably, alpha-disaccharides increased core microbial interactions. The gut microbial composition affected short-chain fatty acids production, with trehalose and cellobiose showing the greatest enhancement. Additionally, beta-disaccharides, particularly cellobiose, were more successful in increasing the butyric acid levels than alpha-disaccharides. Metabolomic profiling indicated that alpha-disaccharides upregulated antioxidative amino acids (e.g., L-proline) and glycerophospholipids, while beta-disaccharides increased delta2-THA, a hepatoprotective fatty acid. This study highlights the differential modulation of gut microbiota and metabolism by disaccharide glycosidic bonds, providing insights into their functional roles.