文献类型：期刊文献

英文题名：A genetically encoded toolkit for tracking live-cell histidine dynamics in space and time

作者：Hu, Hanyang[1,2,3];Gu, Yanfang[1,2,3];Xu, Lei[1,2];Zou, Yejun[1,2,3];Wang, Aoxue[1,2,3];Tao, Rongkun[1,2,3];Chen, Xianjun[1,2,3];Zhao, Yuzheng[1,2,3];Yang, Yi[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, Synthet Biol & Biotechnol Lab, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, CAS Ctr Excellence Brain Sci, Optogenet & Mol Imaging Interdisciplinary Res Ctr, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, 130 Mei Long Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：7

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000395531100001)】；

基金：This research was supported by the 973 Program (2013CB531200 to Y.Y.), NSFC (91313301, 31225008, and 31470833 to Y.Y., 31671484 and 91649123 to Y. Zhao), the Shanghai Science and Technology Commission (14XD1401400 to Y. Y., and 15YF1402600 to Y. Zhao), the Specialized Research Fund for the Doctoral Program of Higher Education (20100074110010 to Y. Y.), the Lift Engineering for Young Talent of China Association for Science and Technology (to Y. Zhao), the State Key Laboratory of Bioreactor Engineering (to Y. Y.), the 111 Project (B07023 to Y. Y.) and the Fundamental Research Funds for the Central Universities (to Y. Y. and Y. Zhao).

语种：英文

摘要：High-resolution spatiotemporal imaging of histidine in single living mammalian cells faces technical challenges. Here, we developed a series of ratiometric, highly responsive, and single fluorescent protein-based histidine sensors of wide dynamic range. We used these sensors to quantify subcellular free-histidine concentrations in glucose-deprived cells and glucose-fed cells. Results showed that cytosolic free-histidine concentration was higher and more sensitive to the environment than free histidine in the mitochondria. Moreover, histidine was readily transported across the plasma membrane and mitochondrial inner membrane, which had almost similar transport rates and transport constants, and histidine transport was not influenced by cellular metabolic state. These sensors are potential tools for tracking histidine dynamics inside subcellular organelles, and they will open an avenue to explore complex histidine signaling.