文献类型：期刊文献

英文题名：Cyclodextrin/chitosan nanoparticles for oral ovalbumin delivery: Preparation, characterization and intestinal mucosal immunity in mice

作者：He, Muye[2];Zhong, Chen[2];Hu, Huibing[2];Jin, Yu[2];Chen, Yanzuo[2];Lou, Kaiyan[2,3];Gao, Feng[1,2,3]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Dept Pharmaceut, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

年份：2019

卷号：14

期号：2

起止页码：193

外文期刊名：ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000462072200008)】；

基金：This work was supported by Science and Technology Commission of Shanghai Municipality (No. 17ZR1406600) and National Science Foundation of China (No. 21577037). This work was also sponsored by Science and Technology Commission of Shanghai Municipality (No. 10DZ2220500 and No. 11DZ2260600).

语种：英文

外文关键词：beta-cyclodextrin; Chitosan nanoparticles; Ovalbumin; Oral protein delivery; Intestinal mucosal immunity

摘要：A novel oral protein delivery system with enhanced intestinal penetration and improved antigen stability based on chitosan (CS) nanoparticles and antigen-cyclodextrin (CD) inclusion complex was prepared by a precipitation/coacervation method. Ovalbumin (OVA) as a model antigen was firstly encapsulated by cyclodextrin, either beta-cyclodextrin (beta-CD) or carboxymethyl-hydroxypropyl-beta-cyclodextrin (CM-HP-beta-CD) and formed OVA-CD inclusion complexes, which were then loaded to chitosan nanoparticles to form OVA loaded beta-CD/CS or CM-HP-beta-CD/CS nanoparticles with uniform particle size (836.3 and 779.2 nm, respectively) and improved OVA loading efficiency (27.6% and 20.4%, respectively). In vitro drug release studies mimicking oral delivery condition of OVA loaded CD/CS nanoparticles showed low initial releases at pH 1.2 for 2h less than 3.0% and a delayed release which was below to 30% at pH 6.8 for further 72 h. More importantly, after oral administration of OVA loaded beta-CD/CS nanoparticles to Balb/c mice, OVA-specific sIgA levels in jejunum of OVA loaded beta-CD/CS nanoparticles were 3.6-fold and 1.9-fold higher than that of OVA solution and OVA loaded chitosan nanoparticles, respectively. In vivo evaluation results showed that OVA loaded CD/CS nanoparticles could enhance its efficacy for inducing intestinal mucosal immune response. In conclusion, our data suggested that CD/CS nanoparticles could serve as a promising antigen-delivery system for oral vaccination. (C) 2018 Published by Elsevier B.V. on behalf of Shenyang Pharmaceutical University.