文献类型：期刊文献

英文题名：Enhanced production of 3-hydroxypropionic acid from glucose via malonyl-CoA pathway by engineered Escherichia coli

作者：Cheng, Zhuan[1];Jiang, Jiaqi[1];Wu, Hui[1];Li, Zhimin[1,2];Ye, Qin[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2016

卷号：200

起止页码：897

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20154801607811);WOS:【SCI-EXPANDED(收录号:WOS:000365811200114)】；

基金：This study was supported by the National Science Foundation for Young Scientist of China (Grant No. 21406065), the Fundamental Research Funds for the Central Universities (Grant Nos. 222201313007, 222201314048 and 22A201514042), the National High Technology Research and Development Program of China (Grant Nos. 2012AA022104 and 2012AA021205). Partially Supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：3-Hydroxypropionate; Acetyl-CoA carboxylase; Malonyl-CoA reductase; Corynebacterium glutamicum; Escherichia coli

摘要：In this study, production of 3-HP via malonyl-CoA was investigated by using metabolically engineered Escherichia coli carrying heterogeneous acetyl-CoA carboxylase (Acc) from Corynebacterium glutamicum and codon-optimized malonyl-CoA reductase (MCR) from Chloroflexus aurantiacus. Three engineered E. coli strains with different host-vector systems were constructed and investigated. The results indicated that the combination of E. coli BL21(DE3) and pET28a was the most efficient host-vector system for 3-HP production, and the highest concentration of 3-HP attained in shake flask cultivation reached 1.80 g/L by the strain BE-MDA with induction at 0.25 mM IPTG and 25 degrees C, and supplementation of NaHCO3 and biotin. In fed-batch fermentation performed in a 5-L reactor, the concentration of 3-HP achieved 10.08 g/L in 36 h. (C) 2015 Elsevier Ltd. All rights reserved.