文献类型：期刊文献

英文题名：Facile Synthesis of Guanidyl-Functionalized Magnetic Polymer Microspheres for Tunable and Specific Capture of Global Phosphopeptides or Only Multiphosphopeptides

作者：Xiong, Zhichao[1];Chen, Yajing[1];Zhang, Lingyi[1];Ren, Jun[1];Zhang, Quanqing[2];Ye, Mingliang[2];Zhang, Weibing[1];Zou, Hanfa[2]

机构：[1]E China Univ Sci & Technol, Dept Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

年份：2014

卷号：6

期号：24

起止页码：22743

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20150100391698);WOS:【SCI-EXPANDED(收录号:WOS:000347139400120)】；

基金：Financial support is gratefully acknowledged from the State Key Program of National Natural Science of China (21235005), the National Natural Science Foundation of China (No.21475044), the National Natural Science Foundation for Young Scientists of China (No.21105027), the Creative Research Group Project by NSFC (2132106), the National Key Scientific Instrument and Equipment Development Project (2012YQ120044), the China State Key Basic Research Program Grant (2013CB911202, 2012CB-910601 and 2012CB-910101), the Analytical Method Innovation Program of MOST (2012IM030900) and the Knowledge Innovation program of DICP to H. F. Zou.

语种：英文

外文关键词：magnetic microspheres; guanidyl; tunable enrichment ability; phosphorylated peptides; mass spectrum

摘要：The highly selective and efficient capture of heterogeneous types of phosphopeptides is critical for comprehensive and in-depth phosphoproteome analysis, but it still remains a challenge since the lack of affinity material with large binding capacity and controllable specificity. Here, a new affinity material was prepared to improve the enrichment capacity and endue the tunable specificity by introducing guanidyl onto poly(glycidyl methacrylate) (PGMA) modified Fe3O4 microsphere (denoted as Fe3O4@PGMA-Guanidyl). The thick polymer shell endows the composite microsphere with large amount of guanidyl and is beneficial to enhancing the affinity interaction between phosphopeptides and the material. Interestingly, the Fe3O4@PGMA-Guanidyl possesses tunable enriching ability for global phosphopeptides or only multiphosphopeptides through simple regulation of buffer composition. The composite has large enrichment capacity (200 mg g(-1)), extremely high detection sensitivity (0.5 fmol), high enrichment recovery (91.30%), great specificity, and rapid magnetic separation. Moreover, the result of the application to capture of phosphopeptides from tryptic digest of nonfat milk has demonstrated the great potential of Fe3O4@PGMA-Guanidyl in detection and identification of low-abundance phosphopeptides of interest in biological sample.