文献类型：期刊文献

英文题名：The c-di-GMP signalling component YfiR regulates multiple bacterial phenotypes and virulence in Pseudomonas plecoglossicida

作者：Wang, Ying[1];Jin, Yinhua[1];Sun, Fei[1];Zhang, Yuanxing[2];Liu, Qin[1,2,3];Wang, Qiyao[1,3];Yang, Dahai[1,3];Zhang, Yibei[1,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Aquat Anim Dis, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2023

卷号：134

期号：7

外文期刊名：JOURNAL OF APPLIED MICROBIOLOGY

收录：;EI(收录号：20242016099480);WOS:【SCI-EXPANDED(收录号:WOS:001039071900001)】；

基金：This work was financially supported by National Natural Science Foundation of China (32102850), China Agriculture Rsearch System of MOF and MARA (CARS-47), and China Postdoctoral Science Foundation (2021M701206).

语种：英文

外文关键词：biofilm; c-di-GMP; large yellow croaker; Pseudomonas plecoglossicida; YfiBNR

摘要：Aims Pseudomonas plecoglossicida (P. plecoglossicida) is the causative agent of visceral granulomas disease in large yellow croaker (Larimichthys crocea) and it causes severe economic loss to its industry. Biofilm formation, related to intracellular cyclic bis (3 & PRIME;-5 & PRIME;) diguanylic acid (c-di-GMP) levels, is essential for the lifestyle of P. plecoglossicida. This research aims to investigate the role of YfiR-a key regulator of the diguanylate cyclase YfiN to regulate c-di-GMP levels and reveal its regulatory function of bacterial virulence expression in P. plecoglossicida. Methods and results A genetic analysis was carried out to identify the yfiBNR operon for c-di-GMP regulation in P. plecoglossicida. Then, we constructed a yfiR mutant and observed increased c-di-GMP levels, enhanced biofilm formation, increased exopolysaccharides, and diminished swimming and swarming motility in this strain. Moreover, through establishing a yolk sac microinjection infection model in zebrafish larvae, an attenuated phenotype of yfiR mutant that manifested as restored survival and lower bacterial colonization was found. Conclusions YfiR is the key regulator of virulence in P. plecoglossicida, which contributes to c-di-GMP level, biofilm formation, exopolysaccharides production, swimming, swarming motility, and bacterial colonization in zebrafish model.