文献类型：期刊文献

英文题名：Direct visualization of mitochondrial redox states via redox index-assisted surface-enhanced Raman spectroscopy enables improved copper-based cancer therapy

作者：Xi, Chengye[1];Wang, Yi[1];Yu, Junjie[1];Zhang, Shiyi[1];Xu, Hanbin[2];He, Yue[1];Wang, Sining[1];Chen, Huaying[3];Xu, Yikai[1];Li, Dawei[1]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn, Key Lab Adv Mat,Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Hunan Normal Univ, Sch Med, Dept Pharm, Key Lab Study & Discovery Small Targeted Mol Hunan, Changsha 410013, Peoples R China;[3]Zhejiang Shuren Univ, Coll Biol & Environm Engn, Hangzhou 310015, Peoples R China

年份：2026

外文期刊名：SCIENCE CHINA-CHEMISTRY

收录：;EI(收录号：20262020717246);WOS:【SCI-EXPANDED(收录号:WOS:001766700700001)】；

基金：This work was supported by the National Natural Science Foundation of China (22504037, 22574050, 22573031, 22402060), the Science and Technology Commission of Shanghai Municipality (24DX1400200, 25ZR1401082), the Program of Introducing Talents of Discipline to Universities (B16017), the Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China (JYB2025XDXM404), and the Fundamental Research Funds for the Central Universities (222201717003). We thank the Research Center of Analysis and Test of East China University of Science and Technology for their help with characterizations.

语种：英文

外文关键词：SERS sensor; copper-induced cell death; mitochondrial redox state; cancer therapy

摘要：Copper cytotoxicity provides a viable way to fight microbial infections and cancer. While it is generally known that redox homeostasis in the mitochondria critically affects copper cytotoxicity, its precise impact remains elusive due to the lack of suitable characterization techniques. Here, we designed a multifunctional plasmonic nanosensor and propose the concept of a redox index, which combined allow dynamic real-time monitoring of the mitochondrial redox state during copper-induced cell death via surface-enhanced Raman spectroscopy. This revealed significant redox swings throughout the cell death process and showed that reactive oxygen species (ROS) significantly elevated copper cytotoxicity. This understanding offers insight into the design of combinatorial therapeutic strategies. For example, we showed that the addition of micromolars of external ROS stimulants improved copper-based cancer treatment efficacy by >6 times. Beyond biological applications, we envisage that our nanosensor will become a useful tool for a wide range of applications based on redox chemistry.