文献类型：期刊文献

英文题名：Characterization of cellular senescence in doxorubicin-induced aging mice

作者：Sun, Tianyue[1];Zhang, Lanxin[1];Feng, Jiali[1];Bao, Lingyuan[1];Wang, Jiqun[1];Song, Zhouzhi[1];Mao, Zhifan[1];Li, Jian[1,2,3];Hu, Zelan[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Frontiers Sci Ctr Mat & Dynam Chem, State Key Lab Bioreactor Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]Dali Univ, Coll Pharm, Yunnan Key Lab Screening & Res Antipathogen Plant, 5 Xue Ren Rd, Dali 671000, Yunnan, Peoples R China;[3]Tongji Univ, Sch Med, Shanghai Peoples Hosp 10, Clin Med Sci & Tech Innovat Ctr, Shanghai 200092, Peoples R China

年份：2022

卷号：163

外文期刊名：EXPERIMENTAL GERONTOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000880173200010)】；

基金：This study was supported by the National Natural Science Foundation of China [22037002, 81772689], the Program for Professor of Special Appointment [Eastern Scholar TP2018025] at Shanghai Institutions of Higher Learning, Sponsored by Natural Science Foundation of Shanghai [21ZR1416700], the Innovation Program of Shanghai Municipal Education Commission, the Innovative Research Team of High-level Local Universities in Shanghai, and the Chinese Special Fund for State Key Laboratory of Bioreactor Engineering [2060204].

语种：英文

外文关键词：Doxorubicin; Senescence; Mice; Metformin

摘要：With a rise in the need to develop anti-aging drugs, a growing number of in vivo studies evaluating the efficacy of potential drug candidates have used doxorubicin-induced aging mice. However, changes in the biomarkers of senescent cells have not been reported in detail in these animals. To lay a foundation for the use of doxorubicin-induced aging mice, we examined the biomarkers of hepatic and renal senescent cells in these mice. We found that the 5 mg/kg doxorubicin dose is optimal to induce cellular senescence in mice. Subsequently, using this dose, we found that doxorubicin-induced an increase in senescence-associated beta-galactosidase (SA-beta-gal) positive cells in the kidney and lipofuscin accumulation in the liver. Some markers of senescent cells (p21(WAF1/CIP1), p16(INK4A), and gamma H2AX) were also significantly upregulated by doxorubicin and then counteracted by metformin treatment. These preliminary findings support the application of doxorubicin-induced aging mice as an animal model to evaluate the efficacy of anti-aging drug candidates.