文献类型：期刊文献

英文题名：Systematic pathway engineering of Corynebacterium glutamicum S9114 for l-ornithine production

作者：Zhang, Bin[1];Yu, Miao[1];Zhou, Ying[1];Li, Yixue[2];Ye, Bang-Ce[1]

机构：[1]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Syst Biol, Shanghai 200031, Peoples R China

年份：2017

卷号：16

外文期刊名：MICROBIAL CELL FACTORIES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000411760100001)】；

基金：This work was supported by Grants from the National Natural Science Foundation of China (31730004 and 21575089).

语种：英文

外文关键词：Corynebacterium glutamicum; l-Ornithine production; Metabolic engineering

摘要：Background: l-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, l-ornithine production is based on microbial fermentation, and few microbes are used for producing l-ornithine owing to unsatisfactory production titer. Results: In this study, Corynebacterium glutamicum S9114, a high glutamate-producing strain, was developed for l-ornithine production by pathway engineering. First, argF was deleted to block l-ornithine to citrulline conversion. To improve l-ornithine production, ncgl1221 encoding glutamate transporter, argR encoding arginine repressor, and putP encoding proline transporter were disrupted. This base strain was further engineered by attenuating oxoglutarate dehydrogenase to increase l-ornithine production. Plasmid-based overexpression of argCJBD operon and lysine/arginine transport protein LysE was tested to strengthen l-ornithine synthesis and transportation. This resulted in efficient l-ornithine production at a titer of 18.4 g/L. Conclusion: These results demonstrate the potential of Corynebacterium glutamicum S9114 for efficient l-ornithine production and provide new targets for strain development.