文献类型：期刊文献

英文题名：Gelling and reducing agents are potential carbon and energy sources in culturing of anaerobic microorganisms

作者：Liu, Yi-Fan[1,2,3];Yang, Liu[1,2,3];He, Qing-Ping[1,2,3];Xu, Yi-Lin[1,2];Zhu, Yu-Tong[1,2];Mi, Yan-Le[1,2];Zhou, Lei[1,2,3];Yang, Shi-Zhong[1,2,3];Gu, Ji-Dong[4];Mu, Bo-Zhong[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr MEOR, Shanghai, Peoples R China;[4]Guangdong Technion Israel Inst Technol, Environm Sci & Engn Grp, Shantou, Guangdong, Peoples R China

年份：2025

卷号：91

期号：3

外文期刊名：APPLIED AND ENVIRONMENTAL MICROBIOLOGY

收录：;EI(收录号：20251318127209);WOS:【SCI-EXPANDED(收录号:WOS:001420106400001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 52074129, 42173076, and 42061134011), Fundamental Research Funds for the Central Universities (No. JKJ01241714), and the Research Program of State Key Laboratory of Bioreactor Engineering to Y.F.L. and B.Z.M and the NSFC/RGC Joint Research Fund (No. 41161160560) to J.D.G.

语种：英文

外文关键词：agar; gellan gum; L-cysteine; Atribacterota; oil reservoir; mangrove soil

摘要：The majority of microorganisms in the environment have yet to be isolated in pure cultures, and the reasons behind this phenomenon remain elusive. In this study, we investigated the possibility of the commonly used gelling agent includ ing agar and gellan gum as a source of carbon and energy in anaerobic roll-tube cultivation from one mangrove sediment sample and two high-temperature oilfield samples. Based on growth tests and genomic evidence, anaerobic gellan degraders were retrieved from genera of Clostridium, Lacrimispora, and lineages from the rarely cultivated phylum Atribacterota. Anaerobic agarolytic microorganisms were found to be members of Bacillus and Clostridium. We also proved the role of carbon and energy sources of L-cysteine, a routine agent used to make culture media anoxic/anaerobic in both enrichment cultures and isolated strains representing Acetomicrobium, Ther modesulfovibrio, Lacrimispora, Clostridium, Bacillus, Coprothermobacter, Citrobacter, and Enterobacter. Furthermore, the isolates and enriched microbial communities utilizing L-cysteine under anaerobic conditions were mainly through L-cysteine desulfuration to pyruvate, ammonia, and sulfide. This study demonstrates that the widely used gelling and reducing agents in the basal medium can serve as carbon and energy sources for anaerobic microorganisms and thus may bias the enrichment and isolation.