文献类型：期刊文献

中文题名：核磁共振氢谱法测定甘露聚糖肽中多糖的含量

英文题名：Determination of Polysaccharide Content in Mannatide by ~1H-NMR

作者：吴梦琪[1];张文清[1];徐志珍[1];宛燕飞[2];张力[2];夏玮[1]

机构：[1]华东理工大学上海市功能性材料化学重点实验室,上海200237;[2]成都利尔药业有限公司,四川成都610083

年份：2017

卷号：36

期号：5

起止页码：693

中文期刊名：分析测试学报

外文期刊名：Journal of Instrumental Analysis

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

语种：中文

中文关键词：核磁共振氢谱;多糖含量;甘露聚糖肽

外文关键词：1 H nuclear magnetic resonance ; polysaccharide content ; mannatide

摘要：以甘露聚糖肽为研究对象,首次采用1H-NMR法测定多糖产品中的多糖含量。以基准试剂邻苯二甲酸氢钾为内标,考察了弛豫延迟时间和采样次数对测定结果的影响。选定核磁共振参数弛豫延迟时间1 s,采样次数16次。考察结果表明,该方法具有很好的重复性和精密度。在对甘露聚糖肽定性鉴定的基础上,采用绝对定量方式测定了甘露聚糖肽实际样品的多糖含量,结果与苯酚-硫酸法一致。该方法专属性高,操作方便,结果准确,不仅可用于甘露聚糖肽中多糖含量的测定,还可用于其它结构明确的多糖含量测定。
A proton-nuclear magnetic resonance（1H -NMR） method was established for the determi- nation of polysaccharide content in mannatide using potassium acid phthalate as the internal standard. Effects of some experiment parameters were investigated. The optimal conditions were selected as fol- lows： relaxation delay time（all）： 1 s; transient number（ns） ： 16. The results indicated that, under the optimal conditions, the good precision and repeatability for the method were obtained. The polysaccharide content in mannatide, determined by the 1H -NMR method with inner standard model, was similar to that of the ultraviolet spectrophotometry method. The established method was simple, specific and repeatable. Therefore, it could be used not only for the determination of polysaccharide content in mannatide, but also for detection of other kinds of polysaccharide with definite structures.