文献类型：期刊文献

中文题名：Ultra-low noise measurements of nanopore-based single molecular detection

英文题名：Ultra-low noise measurements of nanopore-based single molecular detection

作者：Zhen Gu[1];Huifeng Wang[2];Yi-Lun Ying[1];Yi-Tao Long[1]

机构：[1]Key Laboratory for Advanced Materials & School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China;[2]School of Information Science and Engineering, East China University of Science and Technology, Shanghai 200237, China

年份：2017

卷号：62

期号：18

起止页码：1245

中文期刊名：Science Bulletin

外文期刊名：科学通报（英文版）

收录：Scopus;CSCD:【CSCD2017_2018】；PubMed;

基金：supported by the National Natural Science Foundation of China (21327807, 21421004);Innovation Program of Shanghai Municipal Education Commission (2017-01-07-00-02E00023);the Fundamental Research Funds for the Central Universities (222201718001, 222201717003)

语种：英文

中文关键词：Nanopore Membrane capacitance Current amplifier Signal amplifier and recording High current resolution

外文关键词：单分子检测;噪声测量;纳米孔;噪声电压;薄膜电容;仪表系统;电化学技术;密闭空间

摘要：Nanopore is an ultra-sensitive electrochemical technique for single molecular detection in confined space. To suppress the noise in detection of the weak current of nanopore, we investigated the influence of membrane capacitance and applied voltage on the noise of the current signal by model analysis, simulation and experiment. The obtained results demonstrated that membrane capacitance affects the noise by amplifying the noise of the applied voltage. Therefore, suppression of applied voltage noise is an efficient approach for reducing the noise in nanopore detection. Here, we developed an ultra-low noise instrument system for detecting the single molecule signal in nanopores. As demonstrated by nanopore experiments, the p-p noise of the developed system during the recording is reduced to 3.2B pA using the filter of 5 kHz. Therefore, the developed system could be applied in highly sensitive nanopore detection.
Nanopore is an ultra-sensitive electrochemical technique for single molecular detection in confined space.To suppress the noise in detection of the weak current of nanopore,we investigated the influence of membrane capacitance and applied voltage on the noise of the current signal by model analysis,simulation and experiment.The obtained results demonstrated that membrane capacitance affects the noise by amplifying the noise of the applied voltage.Therefore,suppression of applied voltage noise is an efficient approach for reducing the noise in nanopore detection.Here,we developed an ultra-low noise instrument system for detecting the single molecule signal in nanopores.As demonstrated by nanopore experiments,the p-p noise of the developed system during the recording is reduced to 3.26 p A using the filter of 5 k Hz.Therefore,the developed system could be applied in highly sensitive nanopore detection.