文献类型：期刊文献

中文题名：盐霉素自身抗性高产突变菌株筛选

英文题名：Screening of high-salinomycin-yield mutants of Streptomyces albus by self-resistance methodology

作者：刘朋[1];杭海峰[1];储消和[2];郭美锦[1];储炬[1];庄英萍[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心(上海),上海200237;[2]浙江升华拜克生物技术股份有限公司,湖州313220

年份：2012

卷号：37

期号：2

起止页码：101

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：生物反应器国家重点实验室开放课题(20060204);国家支撑项目<抗生素大规模生产关键技术创新--抗生素发酵工艺与装备集成创新研究>(2007 BAI26B02)

语种：中文

中文关键词：盐霉素生物合成;白色链霉菌;梯度平板法;自身抗性筛选

外文关键词：Salinomycin biosynthesis; Streptomyces albus; A gradient plate method; Self-resistance secreening

摘要：目的筛选出具有自身次级代谢产物盐霉素(salinomycin)抗性并且遗传稳定性好的菌株,提高摇瓶效价。方法采用常规化学试剂甲基磺酸乙酯诱变处理盐霉素生产菌株Streptomyces albus S-13孢子,通过系列salinomycin浓度梯度平板进行筛选。结果获得了比生产对照菌株高38%的突变株S-E-6。传代实验表明:S-E-6具有较好的遗传稳定性。同时,通过对盐霉素梯度筛选突变株的单菌落直径与效价进行了统计分析,结果表明突变株菌落直径与盐霉素效价具有正相关特性,线性相关系数(R2)为0.95。
Objective To obtain high-yield and genetic stable mutants of Streptomyces albus using a selfresisitance secreening method. Methods Spores of a Streptomyces albus strain S-13 for industrial production of salinomycin were treated by a chemical mutagen of ethylmethane sulfonate（EMS） and then secreen mutants grown on the salinomycin gradient plates. Results A mutant, designated S-E-6, was successfully selected using a salinomycin gradient plate method. The mean salinomycin titer of S-E-6 was 38% higher than that of the parent strain in flask culture. Also, the production level of the mutant S-E-6 was stable after successive 3-generation subcultures, indicating that the selected mutant has a good genetic stability for salinomycin biosynthesis. Besides, the correlation between the radius of colonies grown on salinomycin gradient plates and their corresponding titers was analyzed, the results show that there is a linear relationship with a correlation coefficient （R2） of 0.9546.