文献类型：期刊文献

中文题名：元素衡算和代谢衡算在金色链霉菌培养过程的应用研究

英文题名：Application of Element and Metablism Balancing for the Cultivation Process with Streptomyces aureofaciens

作者：扶教龙[1];庄英萍[1];黄明志[1];储炬[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2003

卷号：19

期号：4

起止页码：471

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；PubMed;

基金：上海市曙光计划基金资助 (No.0 1SG2 8)~~

语种：中文

中文关键词：元素衡算;代谢衡算;金色链霉菌;培养;发酵

外文关键词：element balancing and metablism balancing, Streptomyces aureofaciens, Chlorotetracycline, fermentation

摘要：应用元素衡算和代谢衡算方法 ,建立了金色链霉菌培养过程的数学模型。利用非线性优化方法对模型中的未知参数进行了估算 ,第一次得到了金霉素 (CTC)生物合成的能量系数为 1 8～ 2 8(molATP·C mol- 1 )。应用该模型对CTC发酵过程中的几个宏观反应进行了预测 ,并与实验值进行了比较。结果表明 ,该模型较好地描述了各个宏观反应速率之间的关系 。
On the base of element and metablism balancing, the mathematical model of the cultivation process with Streptomyces aureofaciens was developed, and the unknown parameters in the model were estimated with the method of nonlinear optimization. Firstly the energetic coefficient of CTC biosynthesis was gained, which was 1 8～2 8 mol-ATP·C-mol -1. The macroscopic reaction rates were predicted in the process and compared with the experimental values.The results show that the model can preferably describe the relationships between several macroscopic reaction rates in the process and can supervise the optimization of CTC fermentation process theoretically.