文献类型：期刊文献

英文题名：Effects of 1,1,1-trichloroethane on enzymatic activity and bacterial community in anaerobic microcosm form sequencing batch reactors

作者：Li, Hui[1,2];Zhang, Wei[1,2];Li, Lu[1,2];Liu, Yong-Di[1];Lin, Kuang-Fei[1,2];Lu, Shu-Guang[1];Mu, Bo-Zhong[3];Du, Xiao-Ming[4];Lu, Qiang[2];Zhang, Qian[3];Shen, Ting-Ting[2];Li, Bing-Zhi[2];Zhao, Li-Ming[3];Li, Yang-Yang[5]

机构：[1]E China Univ Sci & Technol, Sch Resource & Environm Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asse, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Sch Bioengn, Shanghai 200237, Peoples R China;[4]Chinese Res Inst Environm Sci, Dept Soil Pollut Control, Beijing 100012, Peoples R China;[5]Shanghai Res Inst Chem Ind, Shanghai 200062, Peoples R China

年份：2012

卷号：21

期号：5

起止页码：1426

外文期刊名：ECOTOXICOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000305461500014)】；

基金：This research was financially supported by the National Natural Science Foundation of China (40871223, 40901148, 41003031, 41073055), the National Environmental Protection Public Welfare Science and Technology Research Program of China (200909089, 2010467032, 201109013), the Doctoral Program of Higher Education (20110074130002), the Fundamental Research Funds for the Central Universities (WB0911011, WB1014004), the Major Science and Technology Program for Water Pollution Control and Treatment (2009ZX07101-009-03), the National Science Foundation for Postdoctoral Scientists of China (20110490683), and the Postdoctoral Science Foundation of Shanghai (11R21412500). We also would like to thank the anonymous referees for their helpful comments on this paper.

语种：英文

外文关键词：1,1,1-Trichloroethane (TCA); Bacterial community; Enzyme activity; Anaerobic sequencing batch reactor (ASBR); Bioremediation

摘要：1,1,1-Trichloroethane (TCA), a major organic and groundwater contaminant, has very strong toxic effects on humans, plants and microorganisms. Effects of TCA on enzymatic activity and microbial diversity were investigated in the anaerobic sequencing batch reactor (ASBR) under methanogenic, nitrate-reducing, sulfate-reducing and benzene/toluene degrading conditions. The activities of three enzymes (lactate dehydrogenase, phosphatase and protease) were significantly decreased in the presence of 5 mg/L TCA. Within these three affected enzymes, phosphatase activity may serve as a noteworthy marker of bacterial toxicity. The activity of phosphatase was 0.2 U/L in methanogenic conditions with 5 mg/L TCA, which was 99% lower than the controls, and the enzyme activity was 18.6 U/L in methanogenic conditions with 1 mg/L TCA, which was 7% lower than the controls. DGGE profiles showed that TCA altered the bacterial community distribution and diversity obviously during the 21 day of TCA exposure. The enzyme activities decreased second lowest but TCA degrading strains Clostridium sp. DhR-2/LM-G01, Bacterial clone DCE25 and Bacterial clone DPHB06 were enriched in the methanogenic ASBR amended TCA.